Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30191 | 5' | -53.6 | NC_006273.1 | + | 144426 | 0.66 | 0.987431 |
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Target: 5'- --gCGGCGGCGGAcgGUUCG-GGUGGc -3' miRNA: 3'- caaGUCGCCGUCUagCAGGUaCCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 160137 | 0.66 | 0.984096 |
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Target: 5'- --gCGGCGGcCAGcgcuUCGUCCAUaGGUa- -3' miRNA: 3'- caaGUCGCC-GUCu---AGCAGGUA-CCAcu -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 161490 | 0.66 | 0.980129 |
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Target: 5'- --aCAGCGGCAG-UgGUCCAUcucuGUGGc -3' miRNA: 3'- caaGUCGCCGUCuAgCAGGUAc---CACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 164967 | 0.71 | 0.881567 |
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Target: 5'- --gCAGCGGCGG---GUCCAgcUGGUGAc -3' miRNA: 3'- caaGUCGCCGUCuagCAGGU--ACCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 167221 | 0.67 | 0.976459 |
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Target: 5'- -gUCAGCGGCcggugguggcugguaAGGUCGaUCaugggcggCGUGGUGAc -3' miRNA: 3'- caAGUCGCCG---------------UCUAGC-AG--------GUACCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 187741 | 1.09 | 0.007163 |
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Target: 5'- cGUUCAGCGGCAGAUCGUCCAUGGUGAc -3' miRNA: 3'- -CAAGUCGCCGUCUAGCAGGUACCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 188395 | 0.66 | 0.985838 |
|
Target: 5'- -cUCGGCGcaCAGAuccaccgccUCGaCCGUGGUGAa -3' miRNA: 3'- caAGUCGCc-GUCU---------AGCaGGUACCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 208249 | 0.66 | 0.985838 |
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Target: 5'- --gCAGCGGCGG---GUCCAUgaggcgGGUGAu -3' miRNA: 3'- caaGUCGCCGUCuagCAGGUA------CCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 220172 | 0.66 | 0.980129 |
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Target: 5'- --gUAGUGGUugGGGUCGUCCAucUGGaUGGc -3' miRNA: 3'- caaGUCGCCG--UCUAGCAGGU--ACC-ACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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