Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30191 | 5' | -53.6 | NC_006273.1 | + | 132902 | 0.68 | 0.952907 |
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Target: 5'- -cUCGGCGGC-GcgCGcCC-UGGUGAa -3' miRNA: 3'- caAGUCGCCGuCuaGCaGGuACCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 113510 | 0.67 | 0.96705 |
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Target: 5'- --cCGGCGGCGGGUUG-CCcgGGg-- -3' miRNA: 3'- caaGUCGCCGUCUAGCaGGuaCCacu -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 167221 | 0.67 | 0.976459 |
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Target: 5'- -gUCAGCGGCcggugguggcugguaAGGUCGaUCaugggcggCGUGGUGAc -3' miRNA: 3'- caAGUCGCCG---------------UCUAGC-AG--------GUACCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 89293 | 0.66 | 0.985838 |
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Target: 5'- ---aGGCGGCGGGcuuuuUUGUCCcgggGGUGGg -3' miRNA: 3'- caagUCGCCGUCU-----AGCAGGua--CCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 44643 | 0.66 | 0.984096 |
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Target: 5'- cGUUCGGCGGCAGcugguggCGUUCugccUGGaGAa -3' miRNA: 3'- -CAAGUCGCCGUCua-----GCAGGu---ACCaCU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 160137 | 0.66 | 0.984096 |
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Target: 5'- --gCGGCGGcCAGcgcuUCGUCCAUaGGUa- -3' miRNA: 3'- caaGUCGCC-GUCu---AGCAGGUA-CCAcu -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 161490 | 0.66 | 0.980129 |
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Target: 5'- --aCAGCGGCAG-UgGUCCAUcucuGUGGc -3' miRNA: 3'- caaGUCGCCGUCuAgCAGGUAc---CACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 90262 | 0.71 | 0.872306 |
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Target: 5'- -aUCGGCGGCGGcgaAUCGcCCgggcugccccgcagGUGGUGAu -3' miRNA: 3'- caAGUCGCCGUC---UAGCaGG--------------UACCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 188395 | 0.66 | 0.985838 |
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Target: 5'- -cUCGGCGcaCAGAuccaccgccUCGaCCGUGGUGAa -3' miRNA: 3'- caAGUCGCc-GUCU---------AGCaGGUACCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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