Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30208 | 3' | -56.4 | NC_006273.1 | + | 233238 | 0.66 | 0.949206 |
Target: 5'- cGGGGAUuguGAACCGUGGCUgAg----- -3' miRNA: 3'- -CCCCUAu--CUUGGCGCCGGgUagagag -5' |
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30208 | 3' | -56.4 | NC_006273.1 | + | 211132 | 0.66 | 0.949206 |
Target: 5'- gGGGGAcuuuUAGAugUuuggGCGGCCCGcggUUCUa -3' miRNA: 3'- -CCCCU----AUCUugG----CGCCGGGUa--GAGAg -5' |
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30208 | 3' | -56.4 | NC_006273.1 | + | 154445 | 0.66 | 0.953172 |
Target: 5'- uGGGcGAcuc-GCCGCaGCCCGUCgagCUCu -3' miRNA: 3'- -CCC-CUaucuUGGCGcCGGGUAGa--GAG- -5' |
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30208 | 3' | -56.4 | NC_006273.1 | + | 48176 | 0.66 | 0.956558 |
Target: 5'- aGGGGAgcGGAUCGCGGUaaugUCGUUuugacauUCUCu -3' miRNA: 3'- -CCCCUauCUUGGCGCCG----GGUAG-------AGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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