miRNA: 3'- cACUG--UCGGGCgaUGUGGAga--GACAG- -5'
miRNA: 3'- cACUGUCGGGCGAUGUGgagaga---CAG- -5'
miRNA: 3'- cacuGUCGGG--CGAUGUGGAGAgACAG- -5'
miRNA: 3'- -CACUGUCGGGCGAUGUGGAGAGACAG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30213 | 5' | -55.9 | NC_006273.1 | + | 196019 | 0.7 | 0.813469 |
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Target: 5'- uUGGCacGGCCCGgaGCGCCUguugCUGUCu -3' miRNA: 3'- cACUG--UCGGGCgaUGUGGAga--GACAG- -5' |
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| 30213 | 5' | -55.9 | NC_006273.1 | + | 114779 | 0.71 | 0.744295 |
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Target: 5'- aUGGCAGCCCGCUGCGCaacccaaacGUCg -3' miRNA: 3'- cACUGUCGGGCGAUGUGgagaga---CAG- -5' |
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| 30213 | 5' | -55.9 | NC_006273.1 | + | 21987 | 0.74 | 0.581227 |
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Target: 5'- ---uCAGCCCucGCUACGCCUCUaUGUCg -3' miRNA: 3'- cacuGUCGGG--CGAUGUGGAGAgACAG- -5' |
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| 30213 | 5' | -55.9 | NC_006273.1 | + | 201212 | 1.1 | 0.003639 |
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Target: 5'- uGUGACAGCCCGCUACACCUCUCUGUCg -3' miRNA: 3'- -CACUGUCGGGCGAUGUGGAGAGACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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