Results 81 - 87 of 87 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30220 | 5' | -51.3 | NC_006273.1 | + | 102608 | 0.66 | 0.998487 |
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Target: 5'- -cGGCGCGUAaaacgcugggguGUACGgCUAUgguacAGGCGGg -3' miRNA: 3'- acUCGCGCAU------------CAUGC-GGUAa----UCUGCC- -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 131461 | 0.66 | 0.998668 |
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Target: 5'- -cAGCGCGUGuccgcuuucguggcGUACGCCGU--GGCGc -3' miRNA: 3'- acUCGCGCAU--------------CAUGCGGUAauCUGCc -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 140874 | 0.66 | 0.99874 |
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Target: 5'- gGGGCGCGaGGUGCccGCC--UGGcCGGc -3' miRNA: 3'- aCUCGCGCaUCAUG--CGGuaAUCuGCC- -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 167851 | 0.66 | 0.99874 |
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Target: 5'- aGAuGCGUGUGGa--GCCG-UAGACGa -3' miRNA: 3'- aCU-CGCGCAUCaugCGGUaAUCUGCc -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 176696 | 0.66 | 0.99874 |
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Target: 5'- uUGAGCGUGcugcgcgAGUugGUgAccgAGGCGGu -3' miRNA: 3'- -ACUCGCGCa------UCAugCGgUaa-UCUGCC- -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 195754 | 0.66 | 0.99874 |
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Target: 5'- uUGAGCGCGU----CGCCGcUAaaaaagaguGACGGg -3' miRNA: 3'- -ACUCGCGCAucauGCGGUaAU---------CUGCC- -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 175989 | 0.66 | 0.99874 |
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Target: 5'- ---uCGCGUG--ACGCCAgUUGGGCGGc -3' miRNA: 3'- acucGCGCAUcaUGCGGU-AAUCUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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