Results 81 - 87 of 87 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30220 | 5' | -51.3 | NC_006273.1 | + | 15517 | 0.68 | 0.99365 |
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Target: 5'- aGGGCGCGUGGgaGCGUC--UGGACc- -3' miRNA: 3'- aCUCGCGCAUCa-UGCGGuaAUCUGcc -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 15197 | 0.69 | 0.987773 |
|
Target: 5'- -cGGUGcCGU-GUACGCCAUccAGAUGGa -3' miRNA: 3'- acUCGC-GCAuCAUGCGGUAa-UCUGCC- -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 13300 | 0.68 | 0.992707 |
|
Target: 5'- cGAgGCGgGUAGgcUGCCGaacGACGGa -3' miRNA: 3'- aCU-CGCgCAUCauGCGGUaauCUGCC- -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 2290 | 0.71 | 0.949557 |
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Target: 5'- cUGGGCGcCGUguucgGGUACuGCCcgcUGGACGGg -3' miRNA: 3'- -ACUCGC-GCA-----UCAUG-CGGua-AUCUGCC- -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 2240 | 0.69 | 0.982621 |
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Target: 5'- -uGGCGCGUGG-AUGCCGgccgAGACa- -3' miRNA: 3'- acUCGCGCAUCaUGCGGUaa--UCUGcc -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 961 | 0.66 | 0.998487 |
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Target: 5'- cGGGCGUGcUGGgcgccgGCGCCGgua-ACGGg -3' miRNA: 3'- aCUCGCGC-AUCa-----UGCGGUaaucUGCC- -5' |
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| 30220 | 5' | -51.3 | NC_006273.1 | + | 38 | 0.68 | 0.989198 |
|
Target: 5'- gGGGCGCGgcggGGUGggugugUGCCGgguguGGCGGg -3' miRNA: 3'- aCUCGCGCa---UCAU------GCGGUaau--CUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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