Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30221 | 5' | -48.8 | NC_006273.1 | + | 222374 | 0.66 | 0.99959 |
Target: 5'- -aAUUCUACUCGGaaauccgccUugUCCgGAGACGg -3' miRNA: 3'- uaUGAGAUGAGCC---------AugAGG-CUUUGUa -5' |
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30221 | 5' | -48.8 | NC_006273.1 | + | 160219 | 0.67 | 0.999454 |
Target: 5'- -gGgUgUACUCGGUGCUCCagccgcuguaacgcGAAACGg -3' miRNA: 3'- uaUgAgAUGAGCCAUGAGG--------------CUUUGUa -5' |
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30221 | 5' | -48.8 | NC_006273.1 | + | 101873 | 0.68 | 0.998585 |
Target: 5'- -aGCUgUGCUCGGUGCgcaggUCGAugAACAc -3' miRNA: 3'- uaUGAgAUGAGCCAUGa----GGCU--UUGUa -5' |
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30221 | 5' | -48.8 | NC_006273.1 | + | 115577 | 0.68 | 0.998503 |
Target: 5'- gGUGCUgCUGCUguugugggugcggaCGGUGCgggugCCGGGACAg -3' miRNA: 3'- -UAUGA-GAUGA--------------GCCAUGa----GGCUUUGUa -5' |
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30221 | 5' | -48.8 | NC_006273.1 | + | 39165 | 0.7 | 0.991576 |
Target: 5'- -aGCcgggCUGCggCGGUGCUCCGAGcgGCGUu -3' miRNA: 3'- uaUGa---GAUGa-GCCAUGAGGCUU--UGUA- -5' |
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30221 | 5' | -48.8 | NC_006273.1 | + | 206217 | 1.04 | 0.040092 |
Target: 5'- aAUACUCUACUCGGUACUCCGAAACAUc -3' miRNA: 3'- -UAUGAGAUGAGCCAUGAGGCUUUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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