Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30234 | 5' | -55 | NC_006273.1 | + | 196932 | 0.66 | 0.966142 |
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Target: 5'- aCGCGCCgugcgcgaugGCAGGAGCC----GUGgGGu -3' miRNA: 3'- -GCGUGGa---------CGUCCUUGGucaaCACgCC- -5' |
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| 30234 | 5' | -55 | NC_006273.1 | + | 16107 | 0.67 | 0.955863 |
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Target: 5'- aCGC-CCuaUGUGGGuuuaAGCCuaAGUUGUGCGGc -3' miRNA: 3'- -GCGuGG--ACGUCC----UUGG--UCAACACGCC- -5' |
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| 30234 | 5' | -55 | NC_006273.1 | + | 834 | 0.67 | 0.955488 |
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Target: 5'- aGCGCCggggacgggguguUGCGGGcGCUcgggGGUUGgcgGCGGg -3' miRNA: 3'- gCGUGG-------------ACGUCCuUGG----UCAACa--CGCC- -5' |
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| 30234 | 5' | -55 | NC_006273.1 | + | 58155 | 0.68 | 0.943645 |
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Target: 5'- cCGCACCacgGUGGGGAaguaacacucaCGGUUGgGCGGg -3' miRNA: 3'- -GCGUGGa--CGUCCUUg----------GUCAACaCGCC- -5' |
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| 30234 | 5' | -55 | NC_006273.1 | + | 221433 | 0.68 | 0.939127 |
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Target: 5'- aCGCGCCUGUGGaGGGCCAaucGgaccGCGGg -3' miRNA: 3'- -GCGUGGACGUC-CUUGGUcaaCa---CGCC- -5' |
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| 30234 | 5' | -55 | NC_006273.1 | + | 41798 | 0.68 | 0.927873 |
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Target: 5'- gGuCACCUGCGGGGaaacgagacugaaaGCCGcgugacgcuguuGUUGcUGCGGg -3' miRNA: 3'- gC-GUGGACGUCCU--------------UGGU------------CAAC-ACGCC- -5' |
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| 30234 | 5' | -55 | NC_006273.1 | + | 219280 | 1.11 | 0.004101 |
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Target: 5'- cCGCACCUGCAGGAACCAGUUGUGCGGa -3' miRNA: 3'- -GCGUGGACGUCCUUGGUCAACACGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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