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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30240 | 3' | -56.9 | NC_006273.1 | + | 155858 | 0.66 | 0.946429 |
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Target: 5'- uGCgUGCUUCGGUgcgcgUCUCGGUGCuuucGGAa -3' miRNA: 3'- -CG-ACGGAGUCAg----AGAGCCACGca--CCU- -5' |
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| 30240 | 3' | -56.9 | NC_006273.1 | + | 99973 | 0.66 | 0.932797 |
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Target: 5'- cGCUGCC-CAGaUCacacaGGUGCGUGcGAa -3' miRNA: 3'- -CGACGGaGUC-AGagag-CCACGCAC-CU- -5' |
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| 30240 | 3' | -56.9 | NC_006273.1 | + | 101903 | 0.67 | 0.917163 |
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Target: 5'- cCUGCa-CGGUCUCUugcggguugCGGUGCGUGu- -3' miRNA: 3'- cGACGgaGUCAGAGA---------GCCACGCACcu -5' |
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| 30240 | 3' | -56.9 | NC_006273.1 | + | 206489 | 0.68 | 0.886695 |
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Target: 5'- cGCUGCCUCGuuauccggcGUCgcuggcgCUUGGUGCGcgacgaaGGAc -3' miRNA: 3'- -CGACGGAGU---------CAGa------GAGCCACGCa------CCU- -5' |
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| 30240 | 3' | -56.9 | NC_006273.1 | + | 41231 | 0.68 | 0.87996 |
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Target: 5'- cGCUGUUUguGUCaccgCGGcGCGUGGAg -3' miRNA: 3'- -CGACGGAguCAGaga-GCCaCGCACCU- -5' |
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| 30240 | 3' | -56.9 | NC_006273.1 | + | 225141 | 1.11 | 0.003004 |
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Target: 5'- cGCUGCCUCAGUCUCUCGGUGCGUGGAc -3' miRNA: 3'- -CGACGGAGUCAGAGAGCCACGCACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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