miRNA: 3'- caaAGCCGUu--CUUGa-CGU-GACA-GACG- -5'
miRNA: 3'- caaAGCC-GUUCUUGACGUGa----CAgaCG- -5'
miRNA: 3'- -CAAAGCCGU------UCU------------UGACGUGaCaGACG- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30241 | 3' | -49.2 | NC_006273.1 | + | 1890 | 0.67 | 0.998429 |
|
Target: 5'- --gUCGGCAccuGAACcaGCGuCUGUgCUGCg -3' miRNA: 3'- caaAGCCGUu--CUUGa-CGU-GACA-GACG- -5' |
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| 30241 | 3' | -49.2 | NC_006273.1 | + | 1727 | 0.67 | 0.998926 |
|
Target: 5'- --cUCGGaCGGGAGCUGCGCcggcgGUggGCc -3' miRNA: 3'- caaAGCC-GUUCUUGACGUGa----CAgaCG- -5' |
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| 30241 | 3' | -49.2 | NC_006273.1 | + | 1593 | 0.66 | 0.999625 |
|
Target: 5'- gGUUUCGGCAcggcgcGGGcgagguggugcgGCUGUACcG-CUGCa -3' miRNA: 3'- -CAAAGCCGU------UCU------------UGACGUGaCaGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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