Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30250 | 5' | -59.9 | NC_006273.1 | + | 145617 | 0.66 | 0.802054 |
Target: 5'- -cGGUCU--GGGCGGCGGUCgUgguggUGGGg -3' miRNA: 3'- uuUCAGAcgCCCGCCGUCGGgA-----ACCC- -5' |
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30250 | 5' | -59.9 | NC_006273.1 | + | 67984 | 0.66 | 0.827037 |
Target: 5'- gGAAGUCUGUcuGGGCgacgcucugcccGGCGGCgUCaUGGGu -3' miRNA: 3'- -UUUCAGACG--CCCG------------CCGUCG-GGaACCC- -5' |
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30250 | 5' | -59.9 | NC_006273.1 | + | 114092 | 0.66 | 0.827037 |
Target: 5'- ------aGCGGGCGGCGGUgg-UGGGu -3' miRNA: 3'- uuucagaCGCCCGCCGUCGggaACCC- -5' |
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30250 | 5' | -59.9 | NC_006273.1 | + | 187185 | 0.66 | 0.827037 |
Target: 5'- --cGUgUGUGGGC--CGGCCCUcGGGg -3' miRNA: 3'- uuuCAgACGCCCGccGUCGGGAaCCC- -5' |
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30250 | 5' | -59.9 | NC_006273.1 | + | 56279 | 0.66 | 0.835049 |
Target: 5'- -uGGUCUGCugGGGCGGguaCGGCCgc-GGGu -3' miRNA: 3'- uuUCAGACG--CCCGCC---GUCGGgaaCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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