Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30250 | 5' | -59.9 | NC_006273.1 | + | 6481 | 0.67 | 0.78468 |
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Target: 5'- -cAGUgUGCGGGCuccGGCuGCUCgcagUUGGGc -3' miRNA: 3'- uuUCAgACGCCCG---CCGuCGGG----AACCC- -5' |
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| 30250 | 5' | -59.9 | NC_006273.1 | + | 186061 | 0.67 | 0.738244 |
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Target: 5'- --cGUCcgcGCGGGCGGCAGCauccagaCCaUGGa -3' miRNA: 3'- uuuCAGa--CGCCCGCCGUCG-------GGaACCc -5' |
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| 30250 | 5' | -59.9 | NC_006273.1 | + | 145554 | 0.68 | 0.691536 |
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Target: 5'- cGGGGUUc-CGGGCGGCGGUgCUggcgGGGg -3' miRNA: 3'- -UUUCAGacGCCCGCCGUCGgGAa---CCC- -5' |
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| 30250 | 5' | -59.9 | NC_006273.1 | + | 40118 | 0.7 | 0.593765 |
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Target: 5'- --uGUCUGUuugaguccccagGGGaCGGCAGCgcggguCCUUGGGg -3' miRNA: 3'- uuuCAGACG------------CCC-GCCGUCG------GGAACCC- -5' |
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| 30250 | 5' | -59.9 | NC_006273.1 | + | 187185 | 0.66 | 0.827037 |
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Target: 5'- --cGUgUGUGGGC--CGGCCCUcGGGg -3' miRNA: 3'- uuuCAgACGCCCGccGUCGGGAaCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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