Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30251 | 3' | -51.6 | NC_006273.1 | + | 189220 | 0.71 | 0.95297 |
Target: 5'- uGCCGCaGGGcgGUCgcuAGCGUGUcgcagcGUUCGCc -3' miRNA: 3'- -CGGCGaUCC--UAG---UCGCACAa-----CAAGCG- -5' |
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30251 | 3' | -51.6 | NC_006273.1 | + | 22755 | 0.71 | 0.944529 |
Target: 5'- aCCGCUGGGAUgGGUGUuuuUUGUUCu- -3' miRNA: 3'- cGGCGAUCCUAgUCGCAc--AACAAGcg -5' |
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30251 | 3' | -51.6 | NC_006273.1 | + | 143752 | 0.71 | 0.939959 |
Target: 5'- aUUGCUGGGAUCGuuGCG-GUUGU-CGCc -3' miRNA: 3'- cGGCGAUCCUAGU--CGCaCAACAaGCG- -5' |
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30251 | 3' | -51.6 | NC_006273.1 | + | 31664 | 0.75 | 0.792019 |
Target: 5'- aGCUGCgucgucaAGGA-CGGCGUGUUGgaCGCu -3' miRNA: 3'- -CGGCGa------UCCUaGUCGCACAACaaGCG- -5' |
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30251 | 3' | -51.6 | NC_006273.1 | + | 111352 | 0.76 | 0.773614 |
Target: 5'- cGCCGCUGGGuUCAGCGgcgugauccagGUggugGUUgGCg -3' miRNA: 3'- -CGGCGAUCCuAGUCGCa----------CAa---CAAgCG- -5' |
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30251 | 3' | -51.6 | NC_006273.1 | + | 38942 | 1.14 | 0.006078 |
Target: 5'- cGCCGCUAGGAUCAGCGUGUUGUUCGCc -3' miRNA: 3'- -CGGCGAUCCUAGUCGCACAACAAGCG- -5' |
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30251 | 3' | -51.6 | NC_006273.1 | + | 233835 | 1.14 | 0.006078 |
Target: 5'- cGCCGCUAGGAUCAGCGUGUUGUUCGCc -3' miRNA: 3'- -CGGCGAUCCUAGUCGCACAACAAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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