Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30251 | 3' | -51.6 | NC_006273.1 | + | 48111 | 0.68 | 0.987541 |
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Target: 5'- cGCCGCau-GGUCAGCGUGacccucuuUUCGCc -3' miRNA: 3'- -CGGCGaucCUAGUCGCACaac-----AAGCG- -5' |
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| 30251 | 3' | -51.6 | NC_006273.1 | + | 222190 | 0.68 | 0.988987 |
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Target: 5'- cGCCGCacGGGcccgUAGCGggcUGUUuGUUCGCg -3' miRNA: 3'- -CGGCGa-UCCua--GUCGC---ACAA-CAAGCG- -5' |
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| 30251 | 3' | -51.6 | NC_006273.1 | + | 50042 | 0.67 | 0.992555 |
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Target: 5'- aGCCG-UAGGccAUCAGCGUGUcccGUaggUCGUc -3' miRNA: 3'- -CGGCgAUCC--UAGUCGCACAa--CA---AGCG- -5' |
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| 30251 | 3' | -51.6 | NC_006273.1 | + | 229025 | 0.67 | 0.994372 |
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Target: 5'- gGCCGUUGGGGUaCGGCGUaUUccUCGg -3' miRNA: 3'- -CGGCGAUCCUA-GUCGCAcAAcaAGCg -5' |
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| 30251 | 3' | -51.6 | NC_006273.1 | + | 88049 | 0.67 | 0.994688 |
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Target: 5'- aUCGCUGGGcGUCGGCGUccagcacgcgUCGCa -3' miRNA: 3'- cGGCGAUCC-UAGUCGCAcaaca-----AGCG- -5' |
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| 30251 | 3' | -51.6 | NC_006273.1 | + | 191495 | 0.66 | 0.997397 |
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Target: 5'- cGCCGCUuGGAccuUC-GUGcGUUGUcUCGCc -3' miRNA: 3'- -CGGCGAuCCU---AGuCGCaCAACA-AGCG- -5' |
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| 30251 | 3' | -51.6 | NC_006273.1 | + | 233835 | 1.14 | 0.006078 |
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Target: 5'- cGCCGCUAGGAUCAGCGUGUUGUUCGCc -3' miRNA: 3'- -CGGCGAUCCUAGUCGCACAACAAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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