miRNA: 3'- -UACCGGACCauaa------GGUCGAGGCAguC- -5'
miRNA: 3'- uaCCGGacCAuaaGGUCGAGGCAGUc -5'
miRNA: 3'- -UACCGGACC-------------AuaAGGUCGAGGCAGuc -5'
miRNA: 3'- -UACCGGACCAUAAGGUCGAGGCAGUC- -5'
miRNA: 3'- -UACCGGACCAUAAGGUCGAGGCAGUC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30252 | 3' | -55.7 | NC_006273.1 | + | 26656 | 0.66 | 0.94268 |
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Target: 5'- cGUGGCUUGGgaacaccgccUCAGCUCUGUguGg -3' miRNA: 3'- -UACCGGACCauaa------GGUCGAGGCAguC- -5' |
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| 30252 | 3' | -55.7 | NC_006273.1 | + | 75097 | 0.66 | 0.935168 |
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Target: 5'- -cGGCCccGUccaCCAGCUCCGUCGc -3' miRNA: 3'- uaCCGGacCAuaaGGUCGAGGCAGUc -5' |
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| 30252 | 3' | -55.7 | NC_006273.1 | + | 185443 | 0.74 | 0.586462 |
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Target: 5'- cGUGGCCUGGcccgaacgcugcuUcaUCCAGCUCCGUUc- -3' miRNA: 3'- -UACCGGACC-------------AuaAGGUCGAGGCAGuc -5' |
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| 30252 | 3' | -55.7 | NC_006273.1 | + | 234204 | 1.06 | 0.006586 |
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Target: 5'- cAUGGCCUGGUAUUCCAGCUCCGUCAGc -3' miRNA: 3'- -UACCGGACCAUAAGGUCGAGGCAGUC- -5' |
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| 30252 | 3' | -55.7 | NC_006273.1 | + | 39311 | 1.06 | 0.006586 |
|
Target: 5'- cAUGGCCUGGUAUUCCAGCUCCGUCAGc -3' miRNA: 3'- -UACCGGACCAUAAGGUCGAGGCAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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