Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
30253 | 5' | -48.4 | NC_006273.1 | + | 49445 | 0.66 | 0.999883 |
Target: 5'- -uGCU--GUAGGAAGCGuagcCCCGCu- -3' miRNA: 3'- ggUGAuaUAUCCUUCGUuu--GGGCGca -5' |
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30253 | 5' | -48.4 | NC_006273.1 | + | 74829 | 0.66 | 0.999849 |
Target: 5'- aCCGCUcGUAccaGccGCGGGCCCGCGUc -3' miRNA: 3'- -GGUGA-UAUaucCuuCGUUUGGGCGCA- -5' |
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30253 | 5' | -48.4 | NC_006273.1 | + | 153761 | 0.66 | 0.99991 |
Target: 5'- ---aUAUGUGGGAGcGCAAGgCCGCu- -3' miRNA: 3'- ggugAUAUAUCCUU-CGUUUgGGCGca -5' |
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30253 | 5' | -48.4 | NC_006273.1 | + | 83338 | 0.66 | 0.999954 |
Target: 5'- cCgGCgugGUGGGAcccGGCGgcgccguggugggagGACCCGCGg -3' miRNA: 3'- -GgUGauaUAUCCU---UCGU---------------UUGGGCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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