Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
30254 | 3' | -57 | NC_006273.1 | + | 81969 | 0.66 | 0.922597 |
Target: 5'- cGugCCGGugacGAGCAgCGCGCGGaAGGGa -3' miRNA: 3'- -UugGGCCuc--CUUGU-GCGCGUUgUCCC- -5' |
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30254 | 3' | -57 | NC_006273.1 | + | 195485 | 0.66 | 0.927809 |
Target: 5'- cACCUaGGAGGgu-GCGCGCu-CGGGGa -3' miRNA: 3'- uUGGG-CCUCCuugUGCGCGuuGUCCC- -5' |
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30254 | 3' | -57 | NC_006273.1 | + | 196617 | 0.66 | 0.927809 |
Target: 5'- cGCUCGGAcgGGAGCuGCGC-CGGCGGuGGg -3' miRNA: 3'- uUGGGCCU--CCUUG-UGCGcGUUGUC-CC- -5' |
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30254 | 3' | -57 | NC_006273.1 | + | 1126 | 0.66 | 0.932797 |
Target: 5'- cGugCCGGggacgGGGGACuCuuGCGGCGGGGa -3' miRNA: 3'- -UugGGCC-----UCCUUGuGcgCGUUGUCCC- -5' |
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30254 | 3' | -57 | NC_006273.1 | + | 215634 | 0.66 | 0.937562 |
Target: 5'- cACCCaGAuaaGGAuguAgAUGCGCAACAGGa -3' miRNA: 3'- uUGGGcCU---CCU---UgUGCGCGUUGUCCc -5' |
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30254 | 3' | -57 | NC_006273.1 | + | 145309 | 0.66 | 0.942106 |
Target: 5'- cACCUGGuGG-GC-UGCGCcGCGGGGc -3' miRNA: 3'- uUGGGCCuCCuUGuGCGCGuUGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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