Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30254 | 3' | -57 | NC_006273.1 | + | 217429 | 0.72 | 0.671171 |
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Target: 5'- cAGCgCCGGAGGAucguACugGCGCAgAUAGaGGc -3' miRNA: 3'- -UUG-GGCCUCCU----UGugCGCGU-UGUC-CC- -5' |
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| 30254 | 3' | -57 | NC_006273.1 | + | 228708 | 0.68 | 0.873018 |
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Target: 5'- gAACCCGGGuGGGuuguucggaaACG-GCGgGACGGGGa -3' miRNA: 3'- -UUGGGCCU-CCU----------UGUgCGCgUUGUCCC- -5' |
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| 30254 | 3' | -57 | NC_006273.1 | + | 230659 | 0.68 | 0.86515 |
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Target: 5'- gGACCUGGAGaGAGCAgGCGgAccgagccAgAGGGa -3' miRNA: 3'- -UUGGGCCUC-CUUGUgCGCgU-------UgUCCC- -5' |
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| 30254 | 3' | -57 | NC_006273.1 | + | 233685 | 0.68 | 0.87996 |
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Target: 5'- cGCCgCGGcGGAuuuccGCGCGgGgGACGGGGu -3' miRNA: 3'- uUGG-GCCuCCU-----UGUGCgCgUUGUCCC- -5' |
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| 30254 | 3' | -57 | NC_006273.1 | + | 234305 | 0.71 | 0.729326 |
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Target: 5'- uACUCGGAGGGGCGCcCGgCGgcccGCGGGGu -3' miRNA: 3'- uUGGGCCUCCUUGUGcGC-GU----UGUCCC- -5' |
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| 30254 | 3' | -57 | NC_006273.1 | + | 234755 | 1.07 | 0.004868 |
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Target: 5'- gAACCCGGAGGAACACGCGCAACAGGGc -3' miRNA: 3'- -UUGGGCCUCCUUGUGCGCGUUGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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