Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30254 | 3' | -57 | NC_006273.1 | + | 6782 | 0.67 | 0.911507 |
Target: 5'- gAACCgcaGGAGGAGCugACGCuaCAGgAGGGg -3' miRNA: 3'- -UUGGg--CCUCCUUG--UGCGc-GUUgUCCC- -5' |
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30254 | 3' | -57 | NC_006273.1 | + | 1964 | 0.67 | 0.917163 |
Target: 5'- uGCgCGGcGGGGC-CG-GCGACGGGGa -3' miRNA: 3'- uUGgGCCuCCUUGuGCgCGUUGUCCC- -5' |
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30254 | 3' | -57 | NC_006273.1 | + | 1725 | 0.66 | 0.927809 |
Target: 5'- cGCUCGGAcgGGAGCuGCGC-CGGCGGuGGg -3' miRNA: 3'- uUGGGCCU--CCUUG-UGCGcGUUGUC-CC- -5' |
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30254 | 3' | -57 | NC_006273.1 | + | 1126 | 0.66 | 0.932797 |
Target: 5'- cGugCCGGggacgGGGGACuCuuGCGGCGGGGa -3' miRNA: 3'- -UugGGCC-----UCCUUGuGcgCGUUGUCCC- -5' |
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30254 | 3' | -57 | NC_006273.1 | + | 996 | 0.67 | 0.886695 |
Target: 5'- uACCUGGggacAGGGACGgggguUGCGCcgggGACGGGGg -3' miRNA: 3'- uUGGGCC----UCCUUGU-----GCGCG----UUGUCCC- -5' |
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30254 | 3' | -57 | NC_006273.1 | + | 592 | 0.66 | 0.927809 |
Target: 5'- cACCUaGGAGGgu-GCGCGCu-CGGGGa -3' miRNA: 3'- uUGGG-CCUCCuugUGCGCGuuGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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