Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3026 | 5' | -53.6 | NC_001493.1 | + | 84689 | 0.66 | 0.959672 |
Target: 5'- uGGGCGCCCUCagaUCGgcGAcgCUGGAgGg -3' miRNA: 3'- cUUUGCGGGAG---AGCuaCUa-GGCCUgC- -5' |
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3026 | 5' | -53.6 | NC_001493.1 | + | 57275 | 0.66 | 0.959672 |
Target: 5'- uGAGAacaggaGUCCUCUcacugaggCGAUGAUgcCCGGACa -3' miRNA: 3'- -CUUUg-----CGGGAGA--------GCUACUA--GGCCUGc -5' |
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3026 | 5' | -53.6 | NC_001493.1 | + | 109257 | 0.66 | 0.959672 |
Target: 5'- ----gGCCCUgaCGAUGuaCCGGGCGc -3' miRNA: 3'- cuuugCGGGAgaGCUACuaGGCCUGC- -5' |
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3026 | 5' | -53.6 | NC_001493.1 | + | 130768 | 0.66 | 0.96322 |
Target: 5'- -uAACGCaCCacgagCUCGuagagGGUCCGGACc -3' miRNA: 3'- cuUUGCG-GGa----GAGCua---CUAGGCCUGc -5' |
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3026 | 5' | -53.6 | NC_001493.1 | + | 15214 | 0.66 | 0.96322 |
Target: 5'- -uAACGCaCCacgagCUCGuagagGGUCCGGACc -3' miRNA: 3'- cuUUGCG-GGa----GAGCua---CUAGGCCUGc -5' |
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3026 | 5' | -53.6 | NC_001493.1 | + | 72560 | 0.66 | 0.96322 |
Target: 5'- gGGuuCGUUUgCUCGAUGAaaucUCCGGACa -3' miRNA: 3'- -CUuuGCGGGaGAGCUACU----AGGCCUGc -5' |
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3026 | 5' | -53.6 | NC_001493.1 | + | 131264 | 0.66 | 0.96557 |
Target: 5'- ----aGCuCUUCUCGGUGAgcgcaucgagacccUCCGGGCc -3' miRNA: 3'- cuuugCG-GGAGAGCUACU--------------AGGCCUGc -5' |
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3026 | 5' | -53.6 | NC_001493.1 | + | 57419 | 0.66 | 0.966545 |
Target: 5'- -cAGCGCCCU-UCGuu--UCCGGGCa -3' miRNA: 3'- cuUUGCGGGAgAGCuacuAGGCCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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