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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30266 | 5' | -54.9 | NC_006296.2 | + | 12663 | 0.66 | 0.326325 |
Target: 5'- -aGGCCCAUAUaUGGCUUagaGGuGCc -3' miRNA: 3'- gaCCGGGUGUAcACCGAAgagUCuCG- -5' |
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30266 | 5' | -54.9 | NC_006296.2 | + | 1838 | 0.67 | 0.317571 |
Target: 5'- -aGGCCUggaauGCAUcaaGGC-UCUCAGGGCc -3' miRNA: 3'- gaCCGGG-----UGUAca-CCGaAGAGUCUCG- -5' |
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30266 | 5' | -54.9 | NC_006296.2 | + | 12665 | 1.11 | 0.000115 |
Target: 5'- cCUGGCCCACAUGUGGCUUCUCAGAGCc -3' miRNA: 3'- -GACCGGGUGUACACCGAAGAGUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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