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miRNA: 3'- cCCGAGACuCuuCG-GugUa--CACCCGG- -5'
miRNA: 3'- ccCGAGACuCU--------UCGGUGUACA-----CCCGG- -5'
miRNA: 3'- -CCCGAGACUCUUCGGUGUACACCCGG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 30273 | 3' | -55.9 | NC_006296.2 | + | 5012 | 0.66 | 0.331604 |
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Target: 5'- cGGUUCUGcGcuGCuCugGgugGUGGGCCc -3' miRNA: 3'- cCCGAGACuCuuCG-GugUa--CACCCGG- -5' |
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| 30273 | 3' | -55.9 | NC_006296.2 | + | 4736 | 0.72 | 0.112978 |
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Target: 5'- -cGCUCUG-GAugugcagcGGCCGCGUGUuggucGGGCCu -3' miRNA: 3'- ccCGAGACuCU--------UCGGUGUACA-----CCCGG- -5' |
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| 30273 | 3' | -55.9 | NC_006296.2 | + | 12621 | 1.15 | 5.1e-05 |
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Target: 5'- aGGGCUCUGAGAAGCCACAUGUGGGCCa -3' miRNA: 3'- -CCCGAGACUCUUCGGUGUACACCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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