Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30281 | 5' | -49.6 | NC_006324.1 | + | 21422 | 0.66 | 0.866653 |
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Target: 5'- cACA-GAUGGCCCcaagGCGCugauggccuucaAGCAGAuaGGCg -3' miRNA: 3'- -UGUaCUAUUGGGa---CGUG------------UUGUCU--CCG- -5' |
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| 30281 | 5' | -49.6 | NC_006324.1 | + | 28 | 0.67 | 0.819499 |
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Target: 5'- uGCGUGAUGACguugacgugCUGUGCGuaaGGGGGCg -3' miRNA: 3'- -UGUACUAUUGg--------GACGUGUug-UCUCCG- -5' |
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| 30281 | 5' | -49.6 | NC_006324.1 | + | 447 | 0.67 | 0.819499 |
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Target: 5'- uGCGUGAUGACguugacgugCUGUGCGuaaGGGGGCg -3' miRNA: 3'- -UGUACUAUUGg--------GACGUGUug-UCUCCG- -5' |
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| 30281 | 5' | -49.6 | NC_006324.1 | + | 866 | 0.67 | 0.819499 |
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Target: 5'- uGCGUGAUGACguugacgugCUGUGCGuaaGGGGGCg -3' miRNA: 3'- -UGUACUAUUGg--------GACGUGUug-UCUCCG- -5' |
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| 30281 | 5' | -49.6 | NC_006324.1 | + | 33739 | 0.67 | 0.819499 |
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Target: 5'- uGCGUGAUGACguugacgugCUGUGCGuaaGGGGGCg -3' miRNA: 3'- -UGUACUAUUGg--------GACGUGUug-UCUCCG- -5' |
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| 30281 | 5' | -49.6 | NC_006324.1 | + | 34158 | 0.67 | 0.819499 |
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Target: 5'- uGCGUGAUGACguugacgugCUGUGCGuaaGGGGGCg -3' miRNA: 3'- -UGUACUAUUGg--------GACGUGUug-UCUCCG- -5' |
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| 30281 | 5' | -49.6 | NC_006324.1 | + | 22147 | 0.71 | 0.590376 |
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Target: 5'- -aGUGAgGACgCUGCGCcGCAGAGcGCc -3' miRNA: 3'- ugUACUaUUGgGACGUGuUGUCUC-CG- -5' |
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| 30281 | 5' | -49.6 | NC_006324.1 | + | 21213 | 0.73 | 0.442578 |
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Target: 5'- aACGgcGUGACCUUGCACGucugcuuuccaaGCAGGGGUa -3' miRNA: 3'- -UGUacUAUUGGGACGUGU------------UGUCUCCG- -5' |
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| 30281 | 5' | -49.6 | NC_006324.1 | + | 6550 | 1.13 | 0.001053 |
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Target: 5'- gACAUGAUAACCCUGCACAACAGAGGCu -3' miRNA: 3'- -UGUACUAUUGGGACGUGUUGUCUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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