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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 30284 | 5' | -48.6 | NC_006324.1 | + | 20076 | 0.66 | 0.910247 |
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Target: 5'- -aAGU---GGACGCGAUGGAcgAGCCCa -3' miRNA: 3'- gaUCAuagUCUGUGUUGUCU--UCGGGa -5' |
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| 30284 | 5' | -48.6 | NC_006324.1 | + | 7244 | 0.68 | 0.823831 |
|
Target: 5'- aCUGGa---GGACACAGCAGAauacgAGCUCa -3' miRNA: 3'- -GAUCauagUCUGUGUUGUCU-----UCGGGa -5' |
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| 30284 | 5' | -48.6 | NC_006324.1 | + | 12631 | 0.7 | 0.689988 |
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Target: 5'- aUAGUuUgGGGCuACAGCGGcAGCCCUg -3' miRNA: 3'- gAUCAuAgUCUG-UGUUGUCuUCGGGA- -5' |
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| 30284 | 5' | -48.6 | NC_006324.1 | + | 1044 | 0.75 | 0.444529 |
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Target: 5'- ---cUGUCAGGCGC--CAGAAGCCCUu -3' miRNA: 3'- gaucAUAGUCUGUGuuGUCUUCGGGA- -5' |
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| 30284 | 5' | -48.6 | NC_006324.1 | + | 33560 | 0.75 | 0.444529 |
|
Target: 5'- ---cUGUCAGGCGC--CAGAAGCCCUu -3' miRNA: 3'- gaucAUAGUCUGUGuuGUCUUCGGGA- -5' |
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| 30284 | 5' | -48.6 | NC_006324.1 | + | 12877 | 1.09 | 0.002293 |
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Target: 5'- gCUAGUAUCAGACACAACAGAAGCCCUg -3' miRNA: 3'- -GAUCAUAGUCUGUGUUGUCUUCGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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