Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30288 | 3' | -45.9 | NC_006324.1 | + | 31825 | 0.66 | 0.978158 |
Target: 5'- -cAGAGGGGUUGAu-GUACAUGGGu- -3' miRNA: 3'- acUUUUUCCGACUuuCAUGUGCCUgc -5' |
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30288 | 3' | -45.9 | NC_006324.1 | + | 5831 | 0.69 | 0.920705 |
Target: 5'- cGAAc-AGGCUGucggugucaccGUACACGGACu -3' miRNA: 3'- aCUUuuUCCGACuuu--------CAUGUGCCUGc -5' |
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30288 | 3' | -45.9 | NC_006324.1 | + | 9767 | 0.69 | 0.916645 |
Target: 5'- gGggGcuGGCUGAcagaaucaGAGUGCAaaacgcCGGGCGc -3' miRNA: 3'- aCuuUuuCCGACU--------UUCAUGU------GCCUGC- -5' |
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30288 | 3' | -45.9 | NC_006324.1 | + | 25611 | 1.12 | 0.003727 |
Target: 5'- cUGAAAAAGGCUGAAAGUACACGGACGg -3' miRNA: 3'- -ACUUUUUCCGACUUUCAUGUGCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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