Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30289 | 5' | -52.7 | NC_006324.1 | + | 14933 | 0.7 | 0.472291 |
Target: 5'- uUGGUGCgaaaUGCACggCGCACgCUaGCCCg -3' miRNA: 3'- cAUCACGg---AUGUGa-GUGUG-GAaCGGG- -5' |
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30289 | 5' | -52.7 | NC_006324.1 | + | 17639 | 0.66 | 0.723877 |
Target: 5'- aGUAGgucucugugGCCUGCGCgaacUGCACCagGCCg -3' miRNA: 3'- -CAUCa--------CGGAUGUGa---GUGUGGaaCGGg -5' |
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30289 | 5' | -52.7 | NC_006324.1 | + | 18342 | 0.67 | 0.653525 |
Target: 5'- cGUGuUGCCUACguuggcgGCUgGCACCUUGUa- -3' miRNA: 3'- -CAUcACGGAUG-------UGAgUGUGGAACGgg -5' |
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30289 | 5' | -52.7 | NC_006324.1 | + | 27925 | 0.66 | 0.701083 |
Target: 5'- ---aUGCC-GC-CUCAUACUUUGCCUu -3' miRNA: 3'- caucACGGaUGuGAGUGUGGAACGGG- -5' |
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30289 | 5' | -52.7 | NC_006324.1 | + | 27944 | 1.13 | 0.000439 |
Target: 5'- aGUAGUGCCUACACUCACACCUUGCCCg -3' miRNA: 3'- -CAUCACGGAUGUGAGUGUGGAACGGG- -5' |
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30289 | 5' | -52.7 | NC_006324.1 | + | 32914 | 0.66 | 0.717077 |
Target: 5'- gGUGGUGCCauUAUACUCcagagauccaaaucCGCCUUcCCCu -3' miRNA: 3'- -CAUCACGG--AUGUGAGu-------------GUGGAAcGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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