Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30291 | 5' | -56.7 | NC_006324.1 | + | 15060 | 1.1 | 0.000256 |
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Target: 5'- gUUGAUGGCGCGUGCUGCCAACGCACCg -3' miRNA: 3'- -AACUACCGCGCACGACGGUUGCGUGG- -5' |
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| 30291 | 5' | -56.7 | NC_006324.1 | + | 9279 | 0.68 | 0.347326 |
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Target: 5'- gUGGUGGagGCG-GCUGUCuuCgGCGCCg -3' miRNA: 3'- aACUACCg-CGCaCGACGGuuG-CGUGG- -5' |
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| 30291 | 5' | -56.7 | NC_006324.1 | + | 25032 | 0.66 | 0.46217 |
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Target: 5'- ---uUGGgGCaG-GCUGCCGGCGCGUCa -3' miRNA: 3'- aacuACCgCG-CaCGACGGUUGCGUGG- -5' |
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| 30291 | 5' | -56.7 | NC_006324.1 | + | 14996 | 0.66 | 0.472628 |
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Target: 5'- ----gGGCuaGCGUGC-GCCGugcauuuCGCACCa -3' miRNA: 3'- aacuaCCG--CGCACGaCGGUu------GCGUGG- -5' |
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| 30291 | 5' | -56.7 | NC_006324.1 | + | 2043 | 0.66 | 0.46217 |
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Target: 5'- cUGGUGGCGCcgcuUGCgGCCAGCagGCGg- -3' miRNA: 3'- aACUACCGCGc---ACGaCGGUUG--CGUgg -5' |
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| 30291 | 5' | -56.7 | NC_006324.1 | + | 15128 | 0.66 | 0.431552 |
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Target: 5'- gUGGaGGCG-GUGCguugGCagcACGCGCCa -3' miRNA: 3'- aACUaCCGCgCACGa---CGgu-UGCGUGG- -5' |
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| 30291 | 5' | -56.7 | NC_006324.1 | + | 12521 | 0.67 | 0.392641 |
|
Target: 5'- -gGgcGGCagaagGUGUGCUGCCGucuUGCAUCa -3' miRNA: 3'- aaCuaCCG-----CGCACGACGGUu--GCGUGG- -5' |
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| 30291 | 5' | -56.7 | NC_006324.1 | + | 26517 | 0.69 | 0.298054 |
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Target: 5'- gUGGUGGUG-GUGguagccauuauCUGCCAugGCugCu -3' miRNA: 3'- aACUACCGCgCAC-----------GACGGUugCGugG- -5' |
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| 30291 | 5' | -56.7 | NC_006324.1 | + | 14957 | 0.77 | 0.085883 |
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Target: 5'- ----cGGCGCGcaccGCUGCCGuaGCGCGCCu -3' miRNA: 3'- aacuaCCGCGCa---CGACGGU--UGCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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