Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30293 | 3' | -46.5 | NC_006324.1 | + | 6081 | 0.66 | 0.974398 |
Target: 5'- uGaCCCGCCGUAGGUAuaaa-AGggGCg -3' miRNA: 3'- gC-GGGCGGCGUUUAUuucaaUCaaUG- -5' |
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30293 | 3' | -46.5 | NC_006324.1 | + | 28326 | 0.66 | 0.963756 |
Target: 5'- uGCCCuaGCCGCAGcgcuAGUgcGUUAUc -3' miRNA: 3'- gCGGG--CGGCGUUuauuUCAauCAAUG- -5' |
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30293 | 3' | -46.5 | NC_006324.1 | + | 22140 | 0.67 | 0.950354 |
Target: 5'- aCGCUgCGCCGCAGAgcgccGAAGc--GUUACu -3' miRNA: 3'- -GCGG-GCGGCGUUUa----UUUCaauCAAUG- -5' |
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30293 | 3' | -46.5 | NC_006324.1 | + | 11523 | 0.68 | 0.921397 |
Target: 5'- uGCgCGCCGCAGAcAcGGU--GUUGCg -3' miRNA: 3'- gCGgGCGGCGUUUaUuUCAauCAAUG- -5' |
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30293 | 3' | -46.5 | NC_006324.1 | + | 16601 | 1.12 | 0.002995 |
Target: 5'- cCGCCCGCCGCAAAUAAAGUUAGUUACu -3' miRNA: 3'- -GCGGGCGGCGUUUAUUUCAAUCAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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