Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30295 | 5' | -57.6 | NC_006324.1 | + | 17490 | 0.66 | 0.434383 |
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Target: 5'- aGAUGGCGaCuCCAUCGAUGAUGCCc- -3' miRNA: 3'- -CUGCCGCgGcGGUAGCUGUUGUGGau -5' |
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| 30295 | 5' | -57.6 | NC_006324.1 | + | 14930 | 0.67 | 0.386499 |
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Target: 5'- uGCGGCGCCugGCUccgcGUCGGCGGCGugUCUGa -3' miRNA: 3'- cUGCCGCGG--CGG----UAGCUGUUGU--GGAU- -5' |
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| 30295 | 5' | -57.6 | NC_006324.1 | + | 7814 | 0.67 | 0.368344 |
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Target: 5'- aGCGGCGCacgacCGCCAUacuaUGGgAGCACCUu -3' miRNA: 3'- cUGCCGCG-----GCGGUA----GCUgUUGUGGAu -5' |
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| 30295 | 5' | -57.6 | NC_006324.1 | + | 9342 | 0.69 | 0.272078 |
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Target: 5'- aGACGGCGCCGaa---GACAGcCGCCUc -3' miRNA: 3'- -CUGCCGCGGCgguagCUGUU-GUGGAu -5' |
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| 30295 | 5' | -57.6 | NC_006324.1 | + | 16487 | 0.69 | 0.258149 |
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Target: 5'- cGCGGCGCgCGCC-UCGACGAaggggACUUAa -3' miRNA: 3'- cUGCCGCG-GCGGuAGCUGUUg----UGGAU- -5' |
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| 30295 | 5' | -57.6 | NC_006324.1 | + | 18029 | 0.73 | 0.149019 |
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Target: 5'- uGGCGGCGCCGUCuauuUgGAUGGCGCCc- -3' miRNA: 3'- -CUGCCGCGGCGGu---AgCUGUUGUGGau -5' |
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| 30295 | 5' | -57.6 | NC_006324.1 | + | 17982 | 1.06 | 0.000452 |
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Target: 5'- aGACGGCGCCGCCAUCGACAACACCUAc -3' miRNA: 3'- -CUGCCGCGGCGGUAGCUGUUGUGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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