miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30296 5' -58.2 NC_006324.1 + 15453 0.66 0.391276
Target:  5'- ---aGGCGcCCguaucagUGGCGCGGUAGGCg -3'
miRNA:   3'- ugugCCGCcGGa------AUCGCGUCGUUCGa -5'
30296 5' -58.2 NC_006324.1 + 11480 0.66 0.355057
Target:  5'- -uGCGGCGcgcaauccuaacGCCUUGGCGC-GCcuGCa -3'
miRNA:   3'- ugUGCCGC------------CGGAAUCGCGuCGuuCGa -5'
30296 5' -58.2 NC_006324.1 + 15722 0.67 0.329483
Target:  5'- gACAccuuCGGUGGCagCUaAGCGCGGCGcuAGCg -3'
miRNA:   3'- -UGU----GCCGCCG--GAaUCGCGUCGU--UCGa -5'
30296 5' -58.2 NC_006324.1 + 14877 0.67 0.313202
Target:  5'- gACGCGGa-GCCa-GGCGCcGCAGGCg -3'
miRNA:   3'- -UGUGCCgcCGGaaUCGCGuCGUUCGa -5'
30296 5' -58.2 NC_006324.1 + 16551 0.71 0.168386
Target:  5'- -aGCGGCGGCaaUUGGCGCuGCGcccGGCa -3'
miRNA:   3'- ugUGCCGCCGg-AAUCGCGuCGU---UCGa -5'
30296 5' -58.2 NC_006324.1 + 18304 1.07 0.000269
Target:  5'- aACACGGCGGCCUUAGCGCAGCAAGCUg -3'
miRNA:   3'- -UGUGCCGCCGGAAUCGCGUCGUUCGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.