Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30296 | 5' | -58.2 | NC_006324.1 | + | 18304 | 1.07 | 0.000269 |
Target: 5'- aACACGGCGGCCUUAGCGCAGCAAGCUg -3' miRNA: 3'- -UGUGCCGCCGGAAUCGCGUCGUUCGA- -5' |
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30296 | 5' | -58.2 | NC_006324.1 | + | 16551 | 0.71 | 0.168386 |
Target: 5'- -aGCGGCGGCaaUUGGCGCuGCGcccGGCa -3' miRNA: 3'- ugUGCCGCCGg-AAUCGCGuCGU---UCGa -5' |
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30296 | 5' | -58.2 | NC_006324.1 | + | 14877 | 0.67 | 0.313202 |
Target: 5'- gACGCGGa-GCCa-GGCGCcGCAGGCg -3' miRNA: 3'- -UGUGCCgcCGGaaUCGCGuCGUUCGa -5' |
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30296 | 5' | -58.2 | NC_006324.1 | + | 15722 | 0.67 | 0.329483 |
Target: 5'- gACAccuuCGGUGGCagCUaAGCGCGGCGcuAGCg -3' miRNA: 3'- -UGU----GCCGCCG--GAaUCGCGUCGU--UCGa -5' |
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30296 | 5' | -58.2 | NC_006324.1 | + | 11480 | 0.66 | 0.355057 |
Target: 5'- -uGCGGCGcgcaauccuaacGCCUUGGCGC-GCcuGCa -3' miRNA: 3'- ugUGCCGC------------CGGAAUCGCGuCGuuCGa -5' |
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30296 | 5' | -58.2 | NC_006324.1 | + | 15453 | 0.66 | 0.391276 |
Target: 5'- ---aGGCGcCCguaucagUGGCGCGGUAGGCg -3' miRNA: 3'- ugugCCGCcGGa------AUCGCGUCGUUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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