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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30299 | 3' | -44.4 | NC_006324.1 | + | 22812 | 1.14 | 0.003537 |
Target: 5'- aGCGCUUUAGUAGAACUGCUUAACGACu -3' miRNA: 3'- -CGCGAAAUCAUCUUGACGAAUUGCUG- -5' |
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30299 | 3' | -44.4 | NC_006324.1 | + | 16893 | 0.69 | 0.950439 |
Target: 5'- gGCGCgauugccccaguuUUUAaUGGAGCUGCcaAACGACu -3' miRNA: 3'- -CGCG-------------AAAUcAUCUUGACGaaUUGCUG- -5' |
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30299 | 3' | -44.4 | NC_006324.1 | + | 17136 | 0.71 | 0.914766 |
Target: 5'- gGCGCUUUAGUGGAcggaGCcgGUUUuuccACGAUc -3' miRNA: 3'- -CGCGAAAUCAUCU----UGa-CGAAu---UGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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