Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30300 | 3' | -56.2 | NC_006324.1 | + | 33355 | 0.66 | 0.495064 |
Target: 5'- --gUCUGACucGG-CUGUGCCUCCUAa -3' miRNA: 3'- uuaGGACUGc-CCaGAUACGGAGGGUg -5' |
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30300 | 3' | -56.2 | NC_006324.1 | + | 23887 | 1.08 | 0.000425 |
Target: 5'- aAAUCCUGACGGGUCUAUGCCUCCCACa -3' miRNA: 3'- -UUAGGACUGCCCAGAUACGGAGGGUG- -5' |
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30300 | 3' | -56.2 | NC_006324.1 | + | 18107 | 0.7 | 0.28233 |
Target: 5'- --cCCUGccauguuACGGGUCuUAUGCCgcUCCUACg -3' miRNA: 3'- uuaGGAC-------UGCCCAG-AUACGG--AGGGUG- -5' |
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30300 | 3' | -56.2 | NC_006324.1 | + | 14121 | 0.73 | 0.172633 |
Target: 5'- --gCCUGACGGGUCg--GCCUCUa-- -3' miRNA: 3'- uuaGGACUGCCCAGauaCGGAGGgug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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