miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30301 5' -55 NC_006324.1 + 11378 0.66 0.594052
Target:  5'- gAGCGUgCCAGGUauaGAauGGGCGcauGAGGAu -3'
miRNA:   3'- -UCGUAaGGUCCG---CU--CCUGCau-CUCCU- -5'
30301 5' -55 NC_006324.1 + 16905 0.67 0.526573
Target:  5'- cAGCAaccUgCAGGaaaAGGugGUAGAGGGg -3'
miRNA:   3'- -UCGUa--AgGUCCgc-UCCugCAUCUCCU- -5'
30301 5' -55 NC_006324.1 + 15150 0.67 0.515583
Target:  5'- uAGaUAUUCCucgcGCGcGGACGUGGAGGc -3'
miRNA:   3'- -UC-GUAAGGuc--CGCuCCUGCAUCUCCu -5'
30301 5' -55 NC_006324.1 + 24295 1.09 0.000508
Target:  5'- gAGCAUUCCAGGCGAGGACGUAGAGGAg -3'
miRNA:   3'- -UCGUAAGGUCCGCUCCUGCAUCUCCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.