Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30302 | 5' | -55.5 | NC_006324.1 | + | 25221 | 1.09 | 0.000381 |
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Target: 5'- uUUCAGCCAAUUGGCGCGCCUCAGACCu -3' miRNA: 3'- -AAGUCGGUUAACCGCGCGGAGUCUGG- -5' |
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| 30302 | 5' | -55.5 | NC_006324.1 | + | 11498 | 0.67 | 0.460442 |
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Target: 5'- ---cGCC--UUGGCGCGCCUgCAGuCUc -3' miRNA: 3'- aaguCGGuuAACCGCGCGGA-GUCuGG- -5' |
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| 30302 | 5' | -55.5 | NC_006324.1 | + | 11964 | 0.66 | 0.50326 |
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Target: 5'- --aGGCU-GUUGGUguugaaGUGCCUCAGACUg -3' miRNA: 3'- aagUCGGuUAACCG------CGCGGAGUCUGG- -5' |
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| 30302 | 5' | -55.5 | NC_006324.1 | + | 23069 | 0.66 | 0.499987 |
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Target: 5'- cUUGGCCAAuucUgcgucacugacaacUGGCGCGCCcccuUCuGGACCg -3' miRNA: 3'- aAGUCGGUU---A--------------ACCGCGCGG----AG-UCUGG- -5' |
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| 30302 | 5' | -55.5 | NC_006324.1 | + | 15715 | 0.67 | 0.450039 |
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Target: 5'- gUUgGGUUAGa-GGCguccaacacuaGCGCCUCAGACCg -3' miRNA: 3'- -AAgUCGGUUaaCCG-----------CGCGGAGUCUGG- -5' |
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| 30302 | 5' | -55.5 | NC_006324.1 | + | 20624 | 0.67 | 0.450039 |
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Target: 5'- --aAGCCAca-GGCgGCGCUguUCGGACCc -3' miRNA: 3'- aagUCGGUuaaCCG-CGCGG--AGUCUGG- -5' |
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| 30302 | 5' | -55.5 | NC_006324.1 | + | 16935 | 0.68 | 0.38109 |
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Target: 5'- uUUCAGCUu-UUGGCGCaacGCCUgccCAGugCu -3' miRNA: 3'- -AAGUCGGuuAACCGCG---CGGA---GUCugG- -5' |
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| 30302 | 5' | -55.5 | NC_006324.1 | + | 18247 | 0.69 | 0.336351 |
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Target: 5'- ---cGCCA---GGCGCGCCUCcaGCCa -3' miRNA: 3'- aaguCGGUuaaCCGCGCGGAGucUGG- -5' |
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| 30302 | 5' | -55.5 | NC_006324.1 | + | 8872 | 0.7 | 0.319559 |
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Target: 5'- -gCAGCCAccGUUGaGCGCGCUggCAGAg- -3' miRNA: 3'- aaGUCGGU--UAAC-CGCGCGGa-GUCUgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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