Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30304 | 3' | -54.4 | NC_006324.1 | + | 21674 | 0.68 | 0.435962 |
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Target: 5'- -aUCUGCCGCcguuauauCGGCgCCGCGCAGa -3' miRNA: 3'- aaAGACGGUGaac-----GUCG-GGUGUGUCg -5' |
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| 30304 | 3' | -54.4 | NC_006324.1 | + | 27036 | 0.69 | 0.395797 |
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Target: 5'- gUUCUGCCACUUGaucacuGaCCUguACugGGCg -3' miRNA: 3'- aAAGACGGUGAACgu----C-GGG--UGugUCG- -5' |
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| 30304 | 3' | -54.4 | NC_006324.1 | + | 18501 | 0.69 | 0.358098 |
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Target: 5'- aUUUCUGU--CUUGCAgGUCCACgACGGCa -3' miRNA: 3'- -AAAGACGguGAACGU-CGGGUG-UGUCG- -5' |
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| 30304 | 3' | -54.4 | NC_006324.1 | + | 2036 | 0.71 | 0.290508 |
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Target: 5'- ----cGCCGCUUGCGGCCaGCA-GGCg -3' miRNA: 3'- aaagaCGGUGAACGUCGGgUGUgUCG- -5' |
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| 30304 | 3' | -54.4 | NC_006324.1 | + | 20489 | 0.71 | 0.2828 |
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Target: 5'- -gUCUGCUGCgccGCAgcGCCUugGCGGCu -3' miRNA: 3'- aaAGACGGUGaa-CGU--CGGGugUGUCG- -5' |
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| 30304 | 3' | -54.4 | NC_006324.1 | + | 12575 | 0.71 | 0.278252 |
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Target: 5'- --gCUGCCGC-UGUAGCCCcaaacuauuuuaugaACGCGGUu -3' miRNA: 3'- aaaGACGGUGaACGUCGGG---------------UGUGUCG- -5' |
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| 30304 | 3' | -54.4 | NC_006324.1 | + | 26944 | 1.1 | 0.000365 |
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Target: 5'- aUUCUGCCACUUGCAGCCCACACAGCg -3' miRNA: 3'- aAAGACGGUGAACGUCGGGUGUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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