miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30305 3' -51 NC_006324.1 + 5481 0.66 0.771845
Target:  5'- cGCCGCuccauucacaGUGACAGAG-ACAacucugacucGGACAGUg -3'
miRNA:   3'- uUGGCG----------CACUGUUUCgUGU----------UCUGUCA- -5'
30305 3' -51 NC_006324.1 + 133 0.67 0.691821
Target:  5'- gAGCgGCGgGGCGAGGCGCuGAGuCAGg -3'
miRNA:   3'- -UUGgCGCaCUGUUUCGUG-UUCuGUCa -5'
30305 3' -51 NC_006324.1 + 552 0.67 0.691821
Target:  5'- gAGCgGCGgGGCGAGGCGCuGAGuCAGg -3'
miRNA:   3'- -UUGgCGCaCUGUUUCGUG-UUCuGUCa -5'
30305 3' -51 NC_006324.1 + 34053 0.67 0.691821
Target:  5'- gAGCgGCGgGGCGAGGCGCuGAGuCAGg -3'
miRNA:   3'- -UUGgCGCaCUGUUUCGUG-UUCuGUCa -5'
30305 3' -51 NC_006324.1 + 21695 0.73 0.377399
Target:  5'- uGGuuGUguuGUGugGGAGCACAAGGCAGa -3'
miRNA:   3'- -UUggCG---CACugUUUCGUGUUCUGUCa -5'
30305 3' -51 NC_006324.1 + 33537 1.06 0.00195
Target:  5'- aAACCGCGUGACAAAGCACAAGACAGUg -3'
miRNA:   3'- -UUGGCGCACUGUUUCGUGUUCUGUCA- -5'
30305 3' -51 NC_006324.1 + 1068 1.06 0.00195
Target:  5'- aAACCGCGUGACAAAGCACAAGACAGUg -3'
miRNA:   3'- -UUGGCGCACUGUUUCGUGUUCUGUCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.