Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30305 | 5' | -60.5 | NC_006324.1 | + | 17264 | 0.66 | 0.31305 |
Target: 5'- aGUAGUcACUguacCGGCgGCC-CGCCCCGUa -3' miRNA: 3'- -CGUCAcUGA----GUCG-CGGaGCGGGGCGg -5' |
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30305 | 5' | -60.5 | NC_006324.1 | + | 16551 | 0.66 | 0.31305 |
Target: 5'- aGCGGcGGCaaUUGGCGCUgCGCCCgGCa -3' miRNA: 3'- -CGUCaCUG--AGUCGCGGaGCGGGgCGg -5' |
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30305 | 5' | -60.5 | NC_006324.1 | + | 22321 | 0.66 | 0.318474 |
Target: 5'- gGCucGUGAUUCGGCGCCg-GCUcggagcuggacugaCCGCUg -3' miRNA: 3'- -CGu-CACUGAGUCGCGGagCGG--------------GGCGG- -5' |
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30305 | 5' | -60.5 | NC_006324.1 | + | 16617 | 0.67 | 0.2906 |
Target: 5'- uGCAaugccgGGCgCAGCGCCaaUUGCCgCCGCUa -3' miRNA: 3'- -CGUca----CUGaGUCGCGG--AGCGG-GGCGG- -5' |
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30305 | 5' | -60.5 | NC_006324.1 | + | 14941 | 0.68 | 0.243163 |
Target: 5'- cGUAGcGcGCcugCGGCGCCUgGCUCCGCg -3' miRNA: 3'- -CGUCaC-UGa--GUCGCGGAgCGGGGCGg -5' |
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30305 | 5' | -60.5 | NC_006324.1 | + | 3539 | 0.69 | 0.213361 |
Target: 5'- aGCAuUGGCguaaaAGUGCCUUGCCUggCGCCc -3' miRNA: 3'- -CGUcACUGag---UCGCGGAGCGGG--GCGG- -5' |
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30305 | 5' | -60.5 | NC_006324.1 | + | 33993 | 0.98 | 0.001255 |
Target: 5'- ---cUGACUCAGCGCCUCGCCCCGCCg -3' miRNA: 3'- cgucACUGAGUCGCGGAGCGGGGCGG- -5' |
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30305 | 5' | -60.5 | NC_006324.1 | + | 611 | 0.98 | 0.001255 |
Target: 5'- ---cUGACUCAGCGCCUCGCCCCGCCg -3' miRNA: 3'- cgucACUGAGUCGCGGAGCGGGGCGG- -5' |
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30305 | 5' | -60.5 | NC_006324.1 | + | 193 | 0.98 | 0.001255 |
Target: 5'- ---cUGACUCAGCGCCUCGCCCCGCCg -3' miRNA: 3'- cgucACUGAGUCGCGGAGCGGGGCGG- -5' |
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30305 | 5' | -60.5 | NC_006324.1 | + | 33571 | 1.13 | 0.000081 |
Target: 5'- gGCAGUGACUCAGCGCCUCGCCCCGCCg -3' miRNA: 3'- -CGUCACUGAGUCGCGGAGCGGGGCGG- -5' |
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30305 | 5' | -60.5 | NC_006324.1 | + | 1034 | 1.13 | 0.000081 |
Target: 5'- gGCAGUGACUCAGCGCCUCGCCCCGCCg -3' miRNA: 3'- -CGUCACUGAGUCGCGGAGCGGGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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