Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30324 | 3' | -55.1 | NC_006383.2 | + | 14452 | 0.67 | 0.345459 |
Target: 5'- -aAGggGGAGaGAaaucagucaACCCAACaACCGAu -3' miRNA: 3'- ggUCuuCCUC-CUg--------UGGGUUGgUGGCU- -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 12172 | 0.72 | 0.161325 |
Target: 5'- aUAG-AGGAGGugACCgGGUCACCGAu -3' miRNA: 3'- gGUCuUCCUCCugUGGgUUGGUGGCU- -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 11653 | 0.7 | 0.216238 |
Target: 5'- -aAGAGGGAGG-CAUCCAaaGCCGCUc- -3' miRNA: 3'- ggUCUUCCUCCuGUGGGU--UGGUGGcu -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 7213 | 0.71 | 0.181603 |
Target: 5'- gUCAGAGGacgcAGGAaACCCAGCCuCCGAu -3' miRNA: 3'- -GGUCUUCc---UCCUgUGGGUUGGuGGCU- -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 5469 | 0.67 | 0.336511 |
Target: 5'- aCCGGAAGaGGGACccGCCCGagaACguCCGGc -3' miRNA: 3'- -GGUCUUCcUCCUG--UGGGU---UGguGGCU- -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 5290 | 0.72 | 0.139279 |
Target: 5'- cCCAGAA-GAGGACACCCccgcacacuccgaacAcccccucgccccccGCCACCGGg -3' miRNA: 3'- -GGUCUUcCUCCUGUGGG---------------U--------------UGGUGGCU- -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 5118 | 0.68 | 0.302422 |
Target: 5'- aCCAGAGGagccGAGGAgaGCCUGACC-CCGc -3' miRNA: 3'- -GGUCUUC----CUCCUg-UGGGUUGGuGGCu -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 4960 | 1.11 | 0.000145 |
Target: 5'- cCCAGAAGGAGGACACCCAACCACCGAa -3' miRNA: 3'- -GGUCUUCCUCCUGUGGGUUGGUGGCU- -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 4747 | 0.74 | 0.108489 |
Target: 5'- aCAGAaccccaaGGGGGGACcCCCcaaaccaaaggGACCACCGGg -3' miRNA: 3'- gGUCU-------UCCUCCUGuGGG-----------UUGGUGGCU- -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 4709 | 0.7 | 0.198262 |
Target: 5'- gCCAGgcGccGGGCACCCccagacccGCCACCGGc -3' miRNA: 3'- -GGUCuuCcuCCUGUGGGu-------UGGUGGCU- -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 4619 | 0.69 | 0.256379 |
Target: 5'- --cGggGGGGGGCcCCCcGCCuggACCGGg -3' miRNA: 3'- gguCuuCCUCCUGuGGGuUGG---UGGCU- -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 4582 | 0.78 | 0.058301 |
Target: 5'- uCCAGgcGGGGGGCcccccCCCGACCuGCCGGg -3' miRNA: 3'- -GGUCuuCCUCCUGu----GGGUUGG-UGGCU- -5' |
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30324 | 3' | -55.1 | NC_006383.2 | + | 1481 | 0.71 | 0.166195 |
Target: 5'- cCCAG-AGGAGaGACcCCCAGCCaACUGc -3' miRNA: 3'- -GGUCuUCCUC-CUGuGGGUUGG-UGGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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