Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30404 | 5' | -58.8 | NC_006548.1 | + | 23412 | 0.66 | 0.379718 |
Target: 5'- uAACGCUUgGCCAGUGgcagaCUGUAgccCCGGCg -3' miRNA: 3'- -UUGCGAA-UGGUCGCg----GGCAU---GGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 1539 | 0.66 | 0.398015 |
Target: 5'- gAGCGCUaccacuccacUGCCGGCGaCCGccUCGGCg -3' miRNA: 3'- -UUGCGA----------AUGGUCGCgGGCauGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 21981 | 0.66 | 0.407373 |
Target: 5'- uAGCGUcauCCuGCGCCCGaacAUCGGCg -3' miRNA: 3'- -UUGCGaauGGuCGCGGGCa--UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 35867 | 0.66 | 0.398015 |
Target: 5'- uGCGaagaaUGCCGGCGCgauCCGcgcgGCCGGCg -3' miRNA: 3'- uUGCga---AUGGUCGCG---GGCa---UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 28559 | 0.66 | 0.388796 |
Target: 5'- cGGCGC-UACCcGCGacuaCCGUcACCGGUa -3' miRNA: 3'- -UUGCGaAUGGuCGCg---GGCA-UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 12810 | 0.66 | 0.388796 |
Target: 5'- -uUGCUcGCCAGUGCCgCGcgAgCGGCg -3' miRNA: 3'- uuGCGAaUGGUCGCGG-GCa-UgGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 2368 | 0.66 | 0.379718 |
Target: 5'- -cCGCcagGCCGGCGguuuuccaUCCGaGCCGGCUg -3' miRNA: 3'- uuGCGaa-UGGUCGC--------GGGCaUGGCCGA- -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 844 | 0.67 | 0.336501 |
Target: 5'- --gGCUUGCCGGCgGCUCGcuUGCUgGGCUg -3' miRNA: 3'- uugCGAAUGGUCG-CGGGC--AUGG-CCGA- -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 37712 | 0.67 | 0.344851 |
Target: 5'- cACGCUcaucaCAGUGCCgGcgACCGGCg -3' miRNA: 3'- uUGCGAaug--GUCGCGGgCa-UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 1135 | 0.67 | 0.361992 |
Target: 5'- uGCGCg-GCCAGCaacGCCUGUG-CGGCg -3' miRNA: 3'- uUGCGaaUGGUCG---CGGGCAUgGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 2312 | 0.67 | 0.361992 |
Target: 5'- uGCGCgg--CGGCGCCgGagcugACCGGCUa -3' miRNA: 3'- uUGCGaaugGUCGCGGgCa----UGGCCGA- -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 13630 | 0.67 | 0.320247 |
Target: 5'- cAGCaGCUUGCCGcCGCCCGcGCaGGCa -3' miRNA: 3'- -UUG-CGAAUGGUcGCGGGCaUGgCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 4304 | 0.67 | 0.3283 |
Target: 5'- cAACGCc-ACCcGCGCCCugcgcaagGCCGGCa -3' miRNA: 3'- -UUGCGaaUGGuCGCGGGca------UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 11743 | 0.67 | 0.344851 |
Target: 5'- cGGCGCgcGgCGGCGCCgggGUugCGGCg -3' miRNA: 3'- -UUGCGaaUgGUCGCGGg--CAugGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 23884 | 0.68 | 0.304589 |
Target: 5'- cACGUUUGCCAucugccgggucGCGCcaCCGgcaugGCCGGCg -3' miRNA: 3'- uUGCGAAUGGU-----------CGCG--GGCa----UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 5569 | 0.69 | 0.268036 |
Target: 5'- cGCGCagGCCAG-GCCgG-GCCGGCUu -3' miRNA: 3'- uUGCGaaUGGUCgCGGgCaUGGCCGA- -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 4514 | 0.69 | 0.261164 |
Target: 5'- aGGCGCUcgAUCAGCGCCUGgaUGCCGcuuGCg -3' miRNA: 3'- -UUGCGAa-UGGUCGCGGGC--AUGGC---CGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 32150 | 0.69 | 0.254437 |
Target: 5'- --gGCUcgGCCAGCGCCuCGaccuugGCUGGCa -3' miRNA: 3'- uugCGAa-UGGUCGCGG-GCa-----UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 15210 | 0.69 | 0.235107 |
Target: 5'- cGCGCUUGCCGGCcuGCgCGU-CCGGg- -3' miRNA: 3'- uUGCGAAUGGUCG--CGgGCAuGGCCga -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 27169 | 0.69 | 0.238872 |
Target: 5'- -cCGCUUGCacagcagcauugaGGCGCUgCGUACUGGCUg -3' miRNA: 3'- uuGCGAAUGg------------UCGCGG-GCAUGGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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