Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30405 | 3' | -57.6 | NC_006548.1 | + | 12085 | 0.73 | 0.163483 |
Target: 5'- uGCGCUGauGCUCCugcuGCUgGCCGGCGGUUu -3' miRNA: 3'- -CGCGAUggUGAGG----UGA-CGGCCGCUAG- -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 7253 | 0.77 | 0.09272 |
Target: 5'- uGCGCUGCCccugaccggcGCUCUguaGCUGCCGGUcgaGGUCa -3' miRNA: 3'- -CGCGAUGG----------UGAGG---UGACGGCCG---CUAG- -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 4169 | 0.66 | 0.492518 |
Target: 5'- gGCGUggUGCguCuUCCGCacgaaugaacaGCCGGCGAUCg -3' miRNA: 3'- -CGCG--AUGguG-AGGUGa----------CGGCCGCUAG- -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 35867 | 0.66 | 0.471842 |
Target: 5'- uGCGaagaaUGCCGgcgcgaUCCGCgcgGCCGGCGGc- -3' miRNA: 3'- -CGCg----AUGGUg-----AGGUGa--CGGCCGCUag -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 22604 | 0.67 | 0.43187 |
Target: 5'- gGCGCUAUCGUUCCGCUcGCCGaacugccggaaGCGGa- -3' miRNA: 3'- -CGCGAUGGUGAGGUGA-CGGC-----------CGCUag -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 35235 | 0.67 | 0.43187 |
Target: 5'- gGCGCcccggACCAaccggugaaucCUCgACUGgCGGCGAUg -3' miRNA: 3'- -CGCGa----UGGU-----------GAGgUGACgGCCGCUAg -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 2356 | 0.67 | 0.41264 |
Target: 5'- aGCGagGCgCAC-CCGCcagGCCGGCGGUUu -3' miRNA: 3'- -CGCgaUG-GUGaGGUGa--CGGCCGCUAG- -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 22707 | 0.67 | 0.393028 |
Target: 5'- uCGCUGCCAUUCaggcgcagaaggUGCUcgugccgGCCGGCGAUg -3' miRNA: 3'- cGCGAUGGUGAG------------GUGA-------CGGCCGCUAg -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 21492 | 0.67 | 0.392109 |
Target: 5'- aGCGCUccaugagcaaugcACCAUUUCccaucgaucccgaACUGaCGGCGAUCg -3' miRNA: 3'- -CGCGA-------------UGGUGAGG-------------UGACgGCCGCUAG- -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 32776 | 0.68 | 0.341271 |
Target: 5'- gGCGCUGCCgguguggccggACaUCCAgCUGCUGGCaagcGUCa -3' miRNA: 3'- -CGCGAUGG-----------UG-AGGU-GACGGCCGc---UAG- -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 1135 | 0.7 | 0.279263 |
Target: 5'- uGCGCgGCCAgcaacgCCugUG-CGGCGAUCa -3' miRNA: 3'- -CGCGaUGGUga----GGugACgGCCGCUAG- -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 20800 | 0.7 | 0.258433 |
Target: 5'- cUGCUGCCGCgCCagaagGCU-CCGGUGAUCg -3' miRNA: 3'- cGCGAUGGUGaGG-----UGAcGGCCGCUAG- -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 16244 | 0.66 | 0.492518 |
Target: 5'- cGCGC-ACCGCgCCGaaGCCGuGCGGc- -3' miRNA: 3'- -CGCGaUGGUGaGGUgaCGGC-CGCUag -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 22809 | 0.66 | 0.492518 |
Target: 5'- cCGCUucgggacugGCCACUUCAUcGCCGGCc--- -3' miRNA: 3'- cGCGA---------UGGUGAGGUGaCGGCCGcuag -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 13070 | 0.66 | 0.471842 |
Target: 5'- aGCGUcACC-CUCgGCgcgGCCGGUGGc- -3' miRNA: 3'- -CGCGaUGGuGAGgUGa--CGGCCGCUag -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 17511 | 0.66 | 0.471842 |
Target: 5'- -aGCUGCgGCgccaucgCCGCUGCUGGgucgacggcUGAUCg -3' miRNA: 3'- cgCGAUGgUGa------GGUGACGGCC---------GCUAG- -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 2336 | 0.66 | 0.46167 |
Target: 5'- aGCGCUucguuCCugUUCgucgGCUGCgCGGCGGc- -3' miRNA: 3'- -CGCGAu----GGugAGG----UGACG-GCCGCUag -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 28960 | 0.66 | 0.451614 |
Target: 5'- gGCGCcgGCCGcCUCgACgguuugGCCGGUGGc- -3' miRNA: 3'- -CGCGa-UGGU-GAGgUGa-----CGGCCGCUag -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 27400 | 0.67 | 0.422189 |
Target: 5'- --aCUGCCGCUCCuGCUcgGCCaGGCGAg- -3' miRNA: 3'- cgcGAUGGUGAGG-UGA--CGG-CCGCUag -5' |
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30405 | 3' | -57.6 | NC_006548.1 | + | 35950 | 0.67 | 0.41264 |
Target: 5'- uCGUUAUCACgCCGCcgGCCGcGCgGAUCg -3' miRNA: 3'- cGCGAUGGUGaGGUGa-CGGC-CG-CUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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