Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30405 | 3' | -57.6 | NC_006548.1 | + | 16549 | 0.69 | 0.316118 |
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Target: 5'- uGCaGCUGCUGCUCCAggugcuCUGCCgcagccuGGuCGAUCg -3' miRNA: 3'- -CG-CGAUGGUGAGGU------GACGG-------CC-GCUAG- -5' |
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| 30405 | 3' | -57.6 | NC_006548.1 | + | 1135 | 0.7 | 0.279263 |
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Target: 5'- uGCGCgGCCAgcaacgCCugUG-CGGCGAUCa -3' miRNA: 3'- -CGCGaUGGUga----GGugACgGCCGCUAG- -5' |
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| 30405 | 3' | -57.6 | NC_006548.1 | + | 20800 | 0.7 | 0.258433 |
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Target: 5'- cUGCUGCCGCgCCagaagGCU-CCGGUGAUCg -3' miRNA: 3'- cGCGAUGGUGaGG-----UGAcGGCCGCUAG- -5' |
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| 30405 | 3' | -57.6 | NC_006548.1 | + | 16322 | 0.71 | 0.23889 |
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Target: 5'- uCGCgGCCGucgCCACUGgUGGCGAUCu -3' miRNA: 3'- cGCGaUGGUga-GGUGACgGCCGCUAG- -5' |
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| 30405 | 3' | -57.6 | NC_006548.1 | + | 17049 | 0.71 | 0.225957 |
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Target: 5'- aGCGCccgGCUuccagcaGCUCCGCcugcaUGCCGGCGggCa -3' miRNA: 3'- -CGCGa--UGG-------UGAGGUG-----ACGGCCGCuaG- -5' |
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| 30405 | 3' | -57.6 | NC_006548.1 | + | 4460 | 0.71 | 0.220598 |
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Target: 5'- gGCGa-ACCGCUCguCACUGCCGauGUGAUCa -3' miRNA: 3'- -CGCgaUGGUGAG--GUGACGGC--CGCUAG- -5' |
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| 30405 | 3' | -57.6 | NC_006548.1 | + | 12085 | 0.73 | 0.163483 |
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Target: 5'- uGCGCUGauGCUCCugcuGCUgGCCGGCGGUUu -3' miRNA: 3'- -CGCGAUggUGAGG----UGA-CGGCCGCUAG- -5' |
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| 30405 | 3' | -57.6 | NC_006548.1 | + | 7253 | 0.77 | 0.09272 |
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Target: 5'- uGCGCUGCCccugaccggcGCUCUguaGCUGCCGGUcgaGGUCa -3' miRNA: 3'- -CGCGAUGG----------UGAGG---UGACGGCCG---CUAG- -5' |
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| 30405 | 3' | -57.6 | NC_006548.1 | + | 1538 | 1.04 | 0.000806 |
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Target: 5'- aGCGCUACCACUCCACUGCCGGCGAc- -3' miRNA: 3'- -CGCGAUGGUGAGGUGACGGCCGCUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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