Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30405 | 5' | -52.7 | NC_006548.1 | + | 1141 | 0.66 | 0.760695 |
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Target: 5'- aGCGAUGCUGAgcugcgcCGgcuGGGUcgucgacauGGCAuGGAGg -3' miRNA: 3'- -CGCUACGACU-------GCau-UUCG---------CCGU-CCUC- -5' |
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| 30405 | 5' | -52.7 | NC_006548.1 | + | 1504 | 1.12 | 0.000645 |
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Target: 5'- cGCGAUGCUGACGUAAAGCGGCAGGAGg -3' miRNA: 3'- -CGCUACGACUGCAUUUCGCCGUCCUC- -5' |
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| 30405 | 5' | -52.7 | NC_006548.1 | + | 4044 | 0.74 | 0.28784 |
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Target: 5'- -gGAUGUUGACGccauGAGCGaGCAGGAc -3' miRNA: 3'- cgCUACGACUGCau--UUCGC-CGUCCUc -5' |
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| 30405 | 5' | -52.7 | NC_006548.1 | + | 13743 | 0.7 | 0.51326 |
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Target: 5'- cGCGcgGCcugcucgaUGGCGUccAGcCGGUAGGAGa -3' miRNA: 3'- -CGCuaCG--------ACUGCAuuUC-GCCGUCCUC- -5' |
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| 30405 | 5' | -52.7 | NC_006548.1 | + | 15477 | 0.7 | 0.535404 |
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Target: 5'- gGCGGgcugUGCUGACGaGGAGUcuuuccaGCAGGAGu -3' miRNA: 3'- -CGCU----ACGACUGCaUUUCGc------CGUCCUC- -5' |
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| 30405 | 5' | -52.7 | NC_006548.1 | + | 22745 | 0.67 | 0.683996 |
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Target: 5'- gGCGAUGaagUGGCcaGUcccGAAGCGGC-GGAGu -3' miRNA: 3'- -CGCUACg--ACUG--CA---UUUCGCCGuCCUC- -5' |
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| 30405 | 5' | -52.7 | NC_006548.1 | + | 27632 | 0.67 | 0.672565 |
|
Target: 5'- aCGggGUcGGCGgcgAAGGCgaGGCAGGAGu -3' miRNA: 3'- cGCuaCGaCUGCa--UUUCG--CCGUCCUC- -5' |
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| 30405 | 5' | -52.7 | NC_006548.1 | + | 28352 | 0.67 | 0.661092 |
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Target: 5'- cCGGUGaUGACGUAccGCGGUuGGAc -3' miRNA: 3'- cGCUACgACUGCAUuuCGCCGuCCUc -5' |
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| 30405 | 5' | -52.7 | NC_006548.1 | + | 30681 | 0.69 | 0.580604 |
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Target: 5'- uGCGAcgGCgGAC--AGGGUGGCAGGAu -3' miRNA: 3'- -CGCUa-CGaCUGcaUUUCGCCGUCCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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