Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30408 | 3' | -62.2 | NC_006548.1 | + | 2790 | 1.08 | 0.000121 |
Target: 5'- cAGCGUGCUUGCCCUGCGCCGCCUGCAg -3' miRNA: 3'- -UCGCACGAACGGGACGCGGCGGACGU- -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 33327 | 0.74 | 0.05766 |
Target: 5'- cAGCcUGCUguacGCCCUGCGUgGCCgGCAg -3' miRNA: 3'- -UCGcACGAa---CGGGACGCGgCGGaCGU- -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 20328 | 0.74 | 0.066455 |
Target: 5'- cGCGgugacgGCUcUGaccgagaUCCUGCGCCGCCUGCGu -3' miRNA: 3'- uCGCa-----CGA-AC-------GGGACGCGGCGGACGU- -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 32869 | 0.72 | 0.096757 |
Target: 5'- -uCGUGCUggggGCCUUGCGCUG-CUGCGc -3' miRNA: 3'- ucGCACGAa---CGGGACGCGGCgGACGU- -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 12257 | 0.7 | 0.135599 |
Target: 5'- uGGCGUGCUgaUGCCCgcucGC-CCGUCgGCGc -3' miRNA: 3'- -UCGCACGA--ACGGGa---CGcGGCGGaCGU- -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 7123 | 0.7 | 0.135599 |
Target: 5'- aGGCG-GCUcUGCCCcgaguccuUGCGCCGCUUacGCGg -3' miRNA: 3'- -UCGCaCGA-ACGGG--------ACGCGGCGGA--CGU- -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 27277 | 0.68 | 0.164452 |
Target: 5'- cAGCGcG-UUGCUCgGuCGCCGCCUGCu -3' miRNA: 3'- -UCGCaCgAACGGGaC-GCGGCGGACGu -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 2186 | 0.67 | 0.209577 |
Target: 5'- uGCGUGCUcaggaaGUCCUugcGCGCCuUCUGCAg -3' miRNA: 3'- uCGCACGAa-----CGGGA---CGCGGcGGACGU- -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 20248 | 0.67 | 0.226842 |
Target: 5'- aAGUaGUGCUUGCCCggaaccGgGuuGCCUGg- -3' miRNA: 3'- -UCG-CACGAACGGGa-----CgCggCGGACgu -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 1118 | 0.66 | 0.239012 |
Target: 5'- gAGCGaGCcugGCCguCUGCGCgGCCaGCAa -3' miRNA: 3'- -UCGCaCGaa-CGG--GACGCGgCGGaCGU- -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 28682 | 0.66 | 0.245298 |
Target: 5'- cAGCG-GCuUUGUCUU-CGCCGCCUGa- -3' miRNA: 3'- -UCGCaCG-AACGGGAcGCGGCGGACgu -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 2241 | 0.66 | 0.251721 |
Target: 5'- aGGCGUaGCcggucaGCUCcgGCGCCGCCgcGCAg -3' miRNA: 3'- -UCGCA-CGaa----CGGGa-CGCGGCGGa-CGU- -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 496 | 0.66 | 0.258282 |
Target: 5'- ----cGCUUGCUauCUGCgGCCGCCUGgAg -3' miRNA: 3'- ucgcaCGAACGG--GACG-CGGCGGACgU- -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 21096 | 0.66 | 0.262286 |
Target: 5'- uGGCGUccagccugccGCUguucuuccugauaGCUCUGCGCuCGCgCUGCAa -3' miRNA: 3'- -UCGCA----------CGAa------------CGGGACGCG-GCG-GACGU- -5' |
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30408 | 3' | -62.2 | NC_006548.1 | + | 5853 | 0.66 | 0.264983 |
Target: 5'- -aCG-GCcuugGCCUUGCGCgcaGCCUGCAc -3' miRNA: 3'- ucGCaCGaa--CGGGACGCGg--CGGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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