Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30408 | 5' | -55 | NC_006548.1 | + | 13105 | 0.66 | 0.574633 |
Target: 5'- -uCGUUGGU-GUGcUGCAGcUGCAGGUUg -3' miRNA: 3'- uuGCAACCGuCGC-ACGUU-ACGUCCGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 8009 | 0.66 | 0.574633 |
Target: 5'- gAGCGc--GCAGCGcGCcggauGAUGCAGGCg -3' miRNA: 3'- -UUGCaacCGUCGCaCG-----UUACGUCCGg -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 31815 | 0.66 | 0.551915 |
Target: 5'- cAACGUUGuGCcGUGUGCAauuuAUGCGuucGGCg -3' miRNA: 3'- -UUGCAAC-CGuCGCACGU----UACGU---CCGg -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 2799 | 0.66 | 0.551915 |
Target: 5'- -cCGUaccGGCAGCGUGCu-UGCccuGcGCCg -3' miRNA: 3'- uuGCAa--CCGUCGCACGuuACGu--C-CGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 8610 | 0.67 | 0.507381 |
Target: 5'- -gUGUUGGguGaCGUuuccGUggUGCcAGGCCa -3' miRNA: 3'- uuGCAACCguC-GCA----CGuuACG-UCCGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 25177 | 0.67 | 0.496481 |
Target: 5'- cAGCGUUcGUucGGgGUGCAGgaucUGCAGGUCa -3' miRNA: 3'- -UUGCAAcCG--UCgCACGUU----ACGUCCGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 24033 | 0.67 | 0.496481 |
Target: 5'- --aGUUGGCAGUc-GCGAgcgccUGCcAGGCCg -3' miRNA: 3'- uugCAACCGUCGcaCGUU-----ACG-UCCGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 19891 | 0.67 | 0.485687 |
Target: 5'- -----aGGCGGCGUG-AGUGCAGGg- -3' miRNA: 3'- uugcaaCCGUCGCACgUUACGUCCgg -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 27871 | 0.68 | 0.475007 |
Target: 5'- uGGCGggugagGuGCAGC--GCAGUGguGGCCg -3' miRNA: 3'- -UUGCaa----C-CGUCGcaCGUUACguCCGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 28076 | 0.68 | 0.464445 |
Target: 5'- -----aGGCGGCuG-GCGGccUGCAGGCCg -3' miRNA: 3'- uugcaaCCGUCG-CaCGUU--ACGUCCGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 23181 | 0.68 | 0.43352 |
Target: 5'- gGACGUUcggccagcuccGGCAGCGUG--AUGUAcacGGCCa -3' miRNA: 3'- -UUGCAA-----------CCGUCGCACguUACGU---CCGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 600 | 0.68 | 0.43352 |
Target: 5'- uGGCGgcGGCAGCGUcCAAUccucaAGGCCc -3' miRNA: 3'- -UUGCaaCCGUCGCAcGUUAcg---UCCGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 27111 | 0.69 | 0.398044 |
Target: 5'- -uUGUUGuuGCAGCGagGCcgccaccauggcccuGAUGCGGGCCg -3' miRNA: 3'- uuGCAAC--CGUCGCa-CG---------------UUACGUCCGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 36439 | 0.7 | 0.357298 |
Target: 5'- cGCGgcUGaGCAGCGcGUAGuucaucUGCAGGCCg -3' miRNA: 3'- uUGCa-AC-CGUCGCaCGUU------ACGUCCGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 16732 | 0.72 | 0.254658 |
Target: 5'- -uUGUUGGgGGUGaGCAGgcGCAGGCCg -3' miRNA: 3'- uuGCAACCgUCGCaCGUUa-CGUCCGG- -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 34238 | 0.73 | 0.22214 |
Target: 5'- uGCGUgacGGCaacAGCGUGacaGGUGCAGGCa -3' miRNA: 3'- uUGCAa--CCG---UCGCACg--UUACGUCCGg -5' |
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30408 | 5' | -55 | NC_006548.1 | + | 2753 | 1.1 | 0.00039 |
Target: 5'- gAACGUUGGCAGCGUGCAAUGCAGGCCa -3' miRNA: 3'- -UUGCAACCGUCGCACGUUACGUCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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