Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30409 | 3' | -54.5 | NC_006548.1 | + | 7833 | 0.66 | 0.646362 |
Target: 5'- aGACGACAUCGag--CGcAUGAGCuGCCg -3' miRNA: 3'- -CUGUUGUAGCggaaGC-UGCUCG-CGGa -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 29421 | 0.66 | 0.634972 |
Target: 5'- aGCGACAgggUGCCaUCGGCGAgaccGCGCa- -3' miRNA: 3'- cUGUUGUa--GCGGaAGCUGCU----CGCGga -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 20049 | 0.66 | 0.623579 |
Target: 5'- uGACgAGCGggGCC-UCGcUGAGCGCCg -3' miRNA: 3'- -CUG-UUGUagCGGaAGCuGCUCGCGGa -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 24489 | 0.67 | 0.58949 |
Target: 5'- -uUAACAUCaCCUaCGACGAGUGgCUg -3' miRNA: 3'- cuGUUGUAGcGGAaGCUGCUCGCgGA- -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 19910 | 0.67 | 0.58949 |
Target: 5'- cGGC-ACGUCGCUcUCGAUGGuCGCCc -3' miRNA: 3'- -CUGuUGUAGCGGaAGCUGCUcGCGGa -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 25001 | 0.67 | 0.58949 |
Target: 5'- cGugAGCuggaaGUUGCCguacucgucgUUGAUGAGCGCCa -3' miRNA: 3'- -CugUUG-----UAGCGGa---------AGCUGCUCGCGGa -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 1093 | 0.67 | 0.56694 |
Target: 5'- -uCAGCAUCGCUgacgcccaaUUCG-CGAGCgaGCCUg -3' miRNA: 3'- cuGUUGUAGCGG---------AAGCuGCUCG--CGGA- -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 9459 | 0.67 | 0.56694 |
Target: 5'- --gAGCAUCaGCCggUCGAUGAggcguucgGCGCCUg -3' miRNA: 3'- cugUUGUAG-CGGa-AGCUGCU--------CGCGGA- -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 34518 | 0.67 | 0.56694 |
Target: 5'- gGGCAA-GUCGCCgUUCGACG-GCGaCCc -3' miRNA: 3'- -CUGUUgUAGCGG-AAGCUGCuCGC-GGa -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 31853 | 0.67 | 0.555745 |
Target: 5'- aGCAGCGuUCGagguaCaUCGGCGaAGCGCCUg -3' miRNA: 3'- cUGUUGU-AGCg----GaAGCUGC-UCGCGGA- -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 799 | 0.67 | 0.555745 |
Target: 5'- cGACAAgAUCGCCaagcagaugUUCGGCGuG-GCCa -3' miRNA: 3'- -CUGUUgUAGCGG---------AAGCUGCuCgCGGa -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 17534 | 0.67 | 0.555745 |
Target: 5'- cGACAggcuGCAUCG-CUUCGGCcagcugcGGCGCCa -3' miRNA: 3'- -CUGU----UGUAGCgGAAGCUGc------UCGCGGa -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 24038 | 0.68 | 0.544615 |
Target: 5'- gGACGAguUgGCagUCG-CGAGCGCCUg -3' miRNA: 3'- -CUGUUguAgCGgaAGCuGCUCGCGGA- -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 14234 | 0.68 | 0.533559 |
Target: 5'- gGACcAgGUCcggGCCUacaUCGAgGGGCGCCUg -3' miRNA: 3'- -CUGuUgUAG---CGGA---AGCUgCUCGCGGA- -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 22770 | 0.68 | 0.500904 |
Target: 5'- cGGCGGaGUCGCCUUugcggaucgcCGACGAG-GCCg -3' miRNA: 3'- -CUGUUgUAGCGGAA----------GCUGCUCgCGGa -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 20669 | 0.68 | 0.500904 |
Target: 5'- aGCGcCAaCGCCagcUCGACGAGCGCg- -3' miRNA: 3'- cUGUuGUaGCGGa--AGCUGCUCGCGga -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 18440 | 0.68 | 0.490214 |
Target: 5'- aGCGGCGUgGUCUUCuGCaGGGCGCCc -3' miRNA: 3'- cUGUUGUAgCGGAAGcUG-CUCGCGGa -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 21365 | 0.69 | 0.47963 |
Target: 5'- --uGACGUUGCCgccguacUCGACcGGCGCCa -3' miRNA: 3'- cugUUGUAGCGGa------AGCUGcUCGCGGa -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 17483 | 0.7 | 0.428508 |
Target: 5'- cGACGGCugAUCGCCcUCGGCGAacucgguggaagGCGUCg -3' miRNA: 3'- -CUGUUG--UAGCGGaAGCUGCU------------CGCGGa -5' |
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30409 | 3' | -54.5 | NC_006548.1 | + | 2121 | 0.7 | 0.408976 |
Target: 5'- cGACAACAUCGCCgacgCGAUccaGGCGUa- -3' miRNA: 3'- -CUGUUGUAGCGGaa--GCUGc--UCGCGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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