Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30409 | 5' | -55.3 | NC_006548.1 | + | 2905 | 1.12 | 0.000367 |
Target: 5'- cCAAGGCGCUGAUCGACGAGUGCCUCCa -3' miRNA: 3'- -GUUCCGCGACUAGCUGCUCACGGAGG- -5' |
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30409 | 5' | -55.3 | NC_006548.1 | + | 4570 | 0.66 | 0.585054 |
Target: 5'- -cAGGCGCUGAUCGAgcgccUGAGcaccaGCggCCg -3' miRNA: 3'- guUCCGCGACUAGCU-----GCUCa----CGgaGG- -5' |
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30409 | 5' | -55.3 | NC_006548.1 | + | 4771 | 0.71 | 0.335611 |
Target: 5'- -cAGGUGCuUGAgcgUGGCGAucggcaggcuguGUGCCUCCu -3' miRNA: 3'- guUCCGCG-ACUa--GCUGCU------------CACGGAGG- -5' |
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30409 | 5' | -55.3 | NC_006548.1 | + | 5899 | 0.66 | 0.618748 |
Target: 5'- cCAAGGcCGUUccguUCGGCG-GUGCCUUg -3' miRNA: 3'- -GUUCC-GCGAcu--AGCUGCuCACGGAGg -5' |
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30409 | 5' | -55.3 | NC_006548.1 | + | 8191 | 0.7 | 0.370106 |
Target: 5'- uCAuGGCGCaGuUCGGCGAcgGCCUCCc -3' miRNA: 3'- -GUuCCGCGaCuAGCUGCUcaCGGAGG- -5' |
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30409 | 5' | -55.3 | NC_006548.1 | + | 10234 | 0.66 | 0.596257 |
Target: 5'- gGAGGUcuaccGCg---CGAUGAGUGCCgCCa -3' miRNA: 3'- gUUCCG-----CGacuaGCUGCUCACGGaGG- -5' |
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30409 | 5' | -55.3 | NC_006548.1 | + | 12589 | 0.7 | 0.370106 |
Target: 5'- -cAGGCcaGCUGGUCGGCGccGGaUGCCgaggCCa -3' miRNA: 3'- guUCCG--CGACUAGCUGC--UC-ACGGa---GG- -5' |
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30409 | 5' | -55.3 | NC_006548.1 | + | 14473 | 0.71 | 0.35256 |
Target: 5'- gCGAGGCGCUGA-CGGCGA-UGa-UCCa -3' miRNA: 3'- -GUUCCGCGACUaGCUGCUcACggAGG- -5' |
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30409 | 5' | -55.3 | NC_006548.1 | + | 16059 | 0.69 | 0.446004 |
Target: 5'- -uGGGCGCUGGgucUGGCGAuccaugcUGCUUCCg -3' miRNA: 3'- guUCCGCGACUa--GCUGCUc------ACGGAGG- -5' |
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30409 | 5' | -55.3 | NC_006548.1 | + | 32206 | 0.68 | 0.487066 |
Target: 5'- uCGAGGCGCUGGcCGAgcccaGGGUGaUgUCCu -3' miRNA: 3'- -GUUCCGCGACUaGCUg----CUCAC-GgAGG- -5' |
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30409 | 5' | -55.3 | NC_006548.1 | + | 33392 | 0.69 | 0.456092 |
Target: 5'- --uGGUGCUGGUCGACacAGUGgCUgCg -3' miRNA: 3'- guuCCGCGACUAGCUGc-UCACgGAgG- -5' |
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30409 | 5' | -55.3 | NC_006548.1 | + | 34055 | 0.66 | 0.596257 |
Target: 5'- -uGGGCag-GAUCGACGAGgugaacugGCCggCCg -3' miRNA: 3'- guUCCGcgaCUAGCUGCUCa-------CGGa-GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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