Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30413 | 5' | -56.1 | NC_006548.1 | + | 4516 | 0.95 | 0.004617 |
Target: 5'- uACCCGCU-GCCAUCGGCAACAUGAUGa -3' miRNA: 3'- -UGGGCGAcCGGUAGCCGUUGUACUAC- -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 37415 | 0.74 | 0.171635 |
Target: 5'- cACCCucggcCUGGCCgcuaGUCGGCAGCGUGGc- -3' miRNA: 3'- -UGGGc----GACCGG----UAGCCGUUGUACUac -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 23186 | 0.73 | 0.202788 |
Target: 5'- cACCaggaCGUUcGGCCAgcucCGGCAGCGUGAUGu -3' miRNA: 3'- -UGG----GCGA-CCGGUa---GCCGUUGUACUAC- -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 25926 | 0.72 | 0.238734 |
Target: 5'- gGCCCGCgcgGGCCAgcaggCGGUGACAUc--- -3' miRNA: 3'- -UGGGCGa--CCGGUa----GCCGUUGUAcuac -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 23801 | 0.7 | 0.302444 |
Target: 5'- aGCCCGCcGGCCAUgcCGGUGGCGcGAc- -3' miRNA: 3'- -UGGGCGaCCGGUA--GCCGUUGUaCUac -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 17341 | 0.7 | 0.318269 |
Target: 5'- uCCCGCUcGGCC-UUGGCG--GUGAUGu -3' miRNA: 3'- uGGGCGA-CCGGuAGCCGUugUACUAC- -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 33820 | 0.69 | 0.343158 |
Target: 5'- -aUCGcCUGGCCAUCGGCGACc----- -3' miRNA: 3'- ugGGC-GACCGGUAGCCGUUGuacuac -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 22156 | 0.69 | 0.343158 |
Target: 5'- uCCUGCaggaaGGCCAUCGGCAcCAUGc-- -3' miRNA: 3'- uGGGCGa----CCGGUAGCCGUuGUACuac -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 21193 | 0.69 | 0.360514 |
Target: 5'- gGCgCCGCUGGCacguUUGGCGA--UGAUGa -3' miRNA: 3'- -UG-GGCGACCGgu--AGCCGUUguACUAC- -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 30336 | 0.69 | 0.369419 |
Target: 5'- cGCCC-CUGGCCugggCGGCAccgacgccaucuACAUGggGa -3' miRNA: 3'- -UGGGcGACCGGua--GCCGU------------UGUACuaC- -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 22681 | 0.68 | 0.406515 |
Target: 5'- cGCCaCGCUGGCgAUCGaGCAGCu----- -3' miRNA: 3'- -UGG-GCGACCGgUAGC-CGUUGuacuac -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 35850 | 0.68 | 0.425919 |
Target: 5'- gUUCGCcGuGCCGUCGaGCGAUGUGAUGc -3' miRNA: 3'- uGGGCGaC-CGGUAGC-CGUUGUACUAC- -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 35885 | 0.67 | 0.445863 |
Target: 5'- gAUCCGCgcGGCC---GGCGGCGUGAUa -3' miRNA: 3'- -UGGGCGa-CCGGuagCCGUUGUACUAc -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 9439 | 0.67 | 0.445863 |
Target: 5'- aGCUCGC-GGCUAcuugcguccgcUCGGCAACAUGc-- -3' miRNA: 3'- -UGGGCGaCCGGU-----------AGCCGUUGUACuac -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 17100 | 0.67 | 0.456029 |
Target: 5'- cACCC-CcGGCCGUCugGGCAACgAUGAUu -3' miRNA: 3'- -UGGGcGaCCGGUAG--CCGUUG-UACUAc -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 8338 | 0.67 | 0.466319 |
Target: 5'- gAUCCGCaugggccugGGCUAUCuGCAGCAUGcUGa -3' miRNA: 3'- -UGGGCGa--------CCGGUAGcCGUUGUACuAC- -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 30603 | 0.67 | 0.476727 |
Target: 5'- aACCUGC-GGCCGUUGGCGAa--GAa- -3' miRNA: 3'- -UGGGCGaCCGGUAGCCGUUguaCUac -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 12268 | 0.67 | 0.487249 |
Target: 5'- uGCCCGCUcGcCCGUCGGCGcACAgGGc- -3' miRNA: 3'- -UGGGCGAcC-GGUAGCCGU-UGUaCUac -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 27258 | 0.67 | 0.487249 |
Target: 5'- cGCCUGCUGGaCGcgagcacgcUCGGCAuuGUGAUa -3' miRNA: 3'- -UGGGCGACCgGU---------AGCCGUugUACUAc -5' |
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30413 | 5' | -56.1 | NC_006548.1 | + | 30845 | 0.67 | 0.496812 |
Target: 5'- uCCCGCUgggcugcGGCCGUUaugcgGGCAGCAgUGAa- -3' miRNA: 3'- uGGGCGA-------CCGGUAG-----CCGUUGU-ACUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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