Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30414 | 3' | -52.8 | NC_006548.1 | + | 26906 | 0.67 | 0.655288 |
Target: 5'- aUGGGAGAGgACUacggcaaUGGGGUUgCCGCa- -3' miRNA: 3'- cACCUUUUCgUGG-------ACCUCAA-GGCGaa -5' |
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30414 | 3' | -52.8 | NC_006548.1 | + | 23227 | 0.66 | 0.714476 |
Target: 5'- uUGGAGAGGC-CgaGGuGUagCCGCUUg -3' miRNA: 3'- cACCUUUUCGuGgaCCuCAa-GGCGAA- -5' |
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30414 | 3' | -52.8 | NC_006548.1 | + | 18481 | 0.67 | 0.621237 |
Target: 5'- --uGAGAAGCugCUGGuugAGUUUCGCa- -3' miRNA: 3'- cacCUUUUCGugGACC---UCAAGGCGaa -5' |
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30414 | 3' | -52.8 | NC_006548.1 | + | 16485 | 0.71 | 0.410939 |
Target: 5'- -cGGcAGAGCACCUGGAGcagcagCUGCa- -3' miRNA: 3'- caCCuUUUCGUGGACCUCaa----GGCGaa -5' |
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30414 | 3' | -52.8 | NC_006548.1 | + | 14361 | 0.67 | 0.632983 |
Target: 5'- -gGGAAcuGCGCCUGcAGgUCCGCg- -3' miRNA: 3'- caCCUUuuCGUGGACcUCaAGGCGaa -5' |
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30414 | 3' | -52.8 | NC_006548.1 | + | 4646 | 1.05 | 0.001582 |
Target: 5'- cGUGGAAAAGCACCUGGAGUUCCGCUUc -3' miRNA: 3'- -CACCUUUUCGUGGACCUCAAGGCGAA- -5' |
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30414 | 3' | -52.8 | NC_006548.1 | + | 4521 | 0.67 | 0.609501 |
Target: 5'- --cGAucAGCGCCUGGAug-CCGCUUg -3' miRNA: 3'- cacCUuuUCGUGGACCUcaaGGCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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