miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30414 3' -52.8 NC_006548.1 + 26906 0.67 0.655288
Target:  5'- aUGGGAGAGgACUacggcaaUGGGGUUgCCGCa- -3'
miRNA:   3'- cACCUUUUCgUGG-------ACCUCAA-GGCGaa -5'
30414 3' -52.8 NC_006548.1 + 23227 0.66 0.714476
Target:  5'- uUGGAGAGGC-CgaGGuGUagCCGCUUg -3'
miRNA:   3'- cACCUUUUCGuGgaCCuCAa-GGCGAA- -5'
30414 3' -52.8 NC_006548.1 + 18481 0.67 0.621237
Target:  5'- --uGAGAAGCugCUGGuugAGUUUCGCa- -3'
miRNA:   3'- cacCUUUUCGugGACC---UCAAGGCGaa -5'
30414 3' -52.8 NC_006548.1 + 16485 0.71 0.410939
Target:  5'- -cGGcAGAGCACCUGGAGcagcagCUGCa- -3'
miRNA:   3'- caCCuUUUCGUGGACCUCaa----GGCGaa -5'
30414 3' -52.8 NC_006548.1 + 14361 0.67 0.632983
Target:  5'- -gGGAAcuGCGCCUGcAGgUCCGCg- -3'
miRNA:   3'- caCCUUuuCGUGGACcUCaAGGCGaa -5'
30414 3' -52.8 NC_006548.1 + 4646 1.05 0.001582
Target:  5'- cGUGGAAAAGCACCUGGAGUUCCGCUUc -3'
miRNA:   3'- -CACCUUUUCGUGGACCUCAAGGCGAA- -5'
30414 3' -52.8 NC_006548.1 + 4521 0.67 0.609501
Target:  5'- --cGAucAGCGCCUGGAug-CCGCUUg -3'
miRNA:   3'- cacCUuuUCGUGGACCUcaaGGCGAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.