Results 1 - 3 of 3 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30414 | 5' | -54 | NC_006548.1 | + | 12826 | 0.66 | 0.644189 |
Target: 5'- cGCGAGCGGcgcgguaaGUCCGGuGUGGcucGGGCg -3' miRNA: 3'- -CGUUCGUCaa------CAGGCUcCACCa--CCUG- -5' |
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30414 | 5' | -54 | NC_006548.1 | + | 4609 | 1.12 | 0.000459 |
Target: 5'- gGCAAGCAGUUGUCCGAGGUGGUGGACg -3' miRNA: 3'- -CGUUCGUCAACAGGCUCCACCACCUG- -5' |
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30414 | 5' | -54 | NC_006548.1 | + | 24490 | 0.66 | 0.694266 |
Target: 5'- uGCAGGCGGUUGcCCGAGaacgauccGUcuuguugguacuuucGGUGGcCa -3' miRNA: 3'- -CGUUCGUCAACaGGCUC--------CA---------------CCACCuG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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